| 人绒毛膜来源的间充质干细胞成骨成脂分化潜能 |
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| 引用本文： | 陈霞,尹晓娟. 人绒毛膜来源的间充质干细胞成骨成脂分化潜能[J]. 儿科药学杂志, 2011, 17(3): 15-18 |
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| 作者姓名： | 陈霞 尹晓娟 |
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| 作者单位： | 1. 中国人民解放军324医院,重庆,400020
2. 北京军区总医院八一儿童医院,北京,100700 |
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| 基金项目： | 成都军区"十一五"课题"胎盘间充质干细胞治疗新生儿缺氧缺血性脑病的应用研究" |
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| 摘 要： | 目的:探讨人绒毛膜来源的间充质干细胞(hCDMSC)体外生长特性和成骨成脂分化潜能,证实人绒毛膜来源的间充质干细胞作为组织工程种子细胞的可行性.方法:取胎盘组织基蜕膜面用胶原酶和胰蛋白酶法分离培养,通过传代扩增观察细胞形态,MTT法检测细胞增殖曲线,体外向成骨、成脂诱导分化,茜素红和油红O染色鉴定细胞分化能力,RT-P...
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| 关 键 词： | 间充质干细胞 成骨 成脂 分化 |
Osteogenic and Adipogenic Differentiation Potential of Human Chorion-derived Mesenchymal Stem Cells |
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| Chen Xia,Yin Xiaojuan. Osteogenic and Adipogenic Differentiation Potential of Human Chorion-derived Mesenchymal Stem Cells[J]. Journal of Pediatric Pharmacy, 2011, 17(3): 15-18 |
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| Authors: | Chen Xia Yin Xiaojuan |
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| Affiliation: | Chen Xia1,Yin Xiaojuan2 (1.China People's Liberation Army 324 Hospital,Chongqing 400020,China,2.Beijing Military District General Hospital Bayi Children's Hospital,Beijing 100700,China) |
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| Abstract: | Objective:To study the growth characteristics and the potency of osteogenic and adipogenic differentiation of human chorion-derived mesenchymal stem cells(hCDMS) in vitro,to validate whether hCDMS could be used in tissue engineering.Methods: The hCDMSC were isolated from placental basal deciduas by collagens and trypsinase digestion methods.After serial sub cultivation in vitro,the stem cells were introduced.Morphologic appearance of hCDMS was observed and the proliferation rate was measured by MTT assay.Th... |
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| Keywords: | Mesenchymal stem cells Osteogenic Adipogenic Differentiation |
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