血管生成素1在大鼠急性射线皮肤损伤创面愈合过程中的表达

目的研究大鼠急性射线皮肤损伤创面愈合过程中血管生成素1（Ang-1）的表达情况。方法将雄性SD清洁级大鼠54只分为3组。照射组（=24）,直线加速器产生射线（45 Gy）单次照射大鼠臀部皮肤40 mm×40 mm,建立急性射线皮肤损伤模型;烧伤组（=24）,将直径30 mm铜柱于100℃沸水中放置15min,取出后在无外界压力下置于大鼠臀部皮肤8 s,建立深Ⅱ°热力烧伤模型;对照组（=6）,无特殊处理。取不同时期创面皮肤组织,采用免疫组织化学（SP法）检测不同时相点各组大鼠创面组织中Ang-1的表达。结果免疫组化显示,烧伤组在伤后1、2及3周Ang-1表达积分吸光度值均要大于照射组,差异均有统计学意义（〈0.05）。结论急性射线皮肤损伤创面愈合过程中Ang-1持续低表达,不能形成峰值,可能是其损伤创面难愈合的机制之一。

Ang-1 expression in coalescence of acute β-ray radiation skin injury in rats

Objective To investigate the dynamic expression of Ang1 in coalescence among the rats with-ray radiation skin injury.Methods Fifty-four male SD rats were divided into 3 groups.The model of acute radiation skin injury was replicated by 45 Gy-ray to the buttocks skin(Radiation group,= 24).The copper column with 30 mm diameter was placed in boiling water for 15 minutes,and then it was put on the buttocks skin for 8 seconds under the absence of outside pressure after being taken out.After that,the second-degree...