东方田鼠感染血清免疫筛选日本血吸虫成虫cDNA文库的初步报告

东方田鼠 (Microtusfortis,Mf)对日本血吸虫 ( Schistosomajaponicum ,Sj)感染具有抗性。为探讨Mf感染Sj后是否产生针对虫体某些抗原分子的免疫应答 ,作者用Mf受感染血清对Sj成虫cDNA文库进行免疫筛选 ,经初筛和复筛 ,共筛选出 1 2个阳性克隆 ,这些阳性克隆经辅助噬菌体自动剪切后PCR扩增显示 ,插入的SjcDNA片段大小在30 0bp～ 1 .8kb之间 ,其中 30 0bp片段 6个 ,1kb片段 1个 ,1 8kb片段 5个。说明Mf感染血清可识别Sj的特异性抗原分子。后者的免疫保护作用值得进一步研究

Preliminary report on immunoscreening of Schistosoma japonicum adult worm cDNA library by sera from infected Microtus fortis

Microtus fortis(Mf) is resistant to Schistosoma japonicum(Sj) infection. To explore if Mf responses to special Sj antigens after infection, Sj adult worm cDNA library was screened with sera from infected Mf. Twelve positive clones were obtained after rescreening. After automatically excised with the helper phage, positive clones were further identified by PCR. The size of Sj cDNA fragments in positive clones ranged from 300?bp to 1.8?kb. Six of these fragments were 300?bp, one was 1?kb, and five were 1.8?kb in size. The results indicate that the infected Mf serum could recognize Sj specific antigens. It is valuable to further study the immune protection of these cloned molecules.