细胞内肌红蛋白——横纹肌及其肿瘤的特殊标记

肌红蛋白仅存在于横纹肌,被认为是一种胚胎性抗原。从人胸肌提取肌红蛋白,免疫家兔获得抗人肌红蛋白抗血清,对多种正常组织和肿瘤的常规福马林液固定、石蜡包埋材料,进行间接免疫过氧化物酶染色。染色结果仅骨骼肌、心肌和横纹肌肉瘤呈阳性反应。研究6例横纹肌肉瘤,5例阳性、1例可疑阳性。因此可认为肌红蛋白为横纹肌及其肿瘤的一个特殊标记,应用免疫过氧化物酶方法发现瘤细胞胞浆内的肌红蛋白,对于横纹肌肉瘤及有横纹肌分化肿瘤的诊断具有特异性和实用价值。

Intracellular Myoglobin--A Specific Marker for Striated Muscle and Striated Muscle Tumors (An Indirect Immunoperoxidase Study)

Myoglobin is found exclusively in striated muscle. It can be considered a fetal antigen. Myoglobin was prepared from normal human thoracic muscle and was used to immunize the rabbits to obtain antimyoglobin antisera. The indirecf immunoperoxidase reaction was performed on numerous normal tissues and a variety of tumors. All the tissues were paraffin-embedded and formalin-fixed. The resuits from skeletal and cardiac muscle and rhabdomyosa-rcoma were positive. Of six cases of rhabdomyosarcoma, five were positive; one was undefined. Therefore, myoglobin should be considered as a specific marker for striated muscle and rhabdomyosarcoma, and the immunoperoxidase technique may be a useful method for the diagnosis of rhabdomyosarcom and other neoplasamas with rhabdomyoblastic differentiation.