| 乙肝病毒表面抗原基因疫苗诱导小鼠细胞及体液免疫应答的研究 |
| |
| 引用本文： | 刘双虎,谭德明,李聪智,燕美玉,孙科柱.乙肝病毒表面抗原基因疫苗诱导小鼠细胞及体液免疫应答的研究[J].中南大学学报(医学版),1999(4). |
| |
| 作者姓名： | 刘双虎 谭德明 李聪智 燕美玉 孙科柱 |
| |
| 作者单位： | Institute of Infectious Diseases，Xiangya Hospital，Hunan Medical University，Changsha 410008 |
| |
| 摘 要： | 为观察乙肝病毒 Ｈ Ｂｓ Ａｇ 基因疫苗免疫小鼠后的细胞及体液免疫应答，将该疫苗定向克隆于质粒ｐｃ Ｄ Ｎ Ａ３ 巨细胞病毒启动子下游，构建能在真核细胞内表达的重组基因疫苗ｐｃ Ｄ Ｎ Ａ Ｈ Ｂｓ Ａｇ 。将此疫苗通过多点肌肉注射免疫 Ｂａｌｂ／ Ｃ 小鼠后第六周，实验组小鼠均出现抗－ Ｈ Ｂｓ Ｉｇ Ｇ 阳性，其抗体水平明显高于对照组，同时 Ｔｈ１ 免疫应答相关的细胞因子 Ｉ Ｌ２ 及γ干扰素水平也明显高于对照组，表明本文构建的基因疫苗ｐｃ Ｄ Ｎ Ａ３ Ｈ Ｂｓ Ａｇ 能诱导 Ｂａｌｂ／ Ｃ 小鼠产生良好的细胞及体液免疫应答。
|
| 关 键 词： | 乙型肝炎病毒 基因疫苗 DNA 小鼠 |
Specific cellular and humoral immune responses induced by intramuscular injection of DNA vaccine containing HBV HBsAg gene in mice |
| |
| Abstract: | To know the influences on the function of cellular and humoral immunity in mice by DNA vaccine containing HBV HBsAg gene, the DNA vaccine, pcDNA3 HBsAg, in which the HBsAg gene sequences of HBV were inserted into downstream of CMV promoter in pcDNA3, were injected intramuscularly into Balb/C mice. The results were that the serum anti HBsIgG was detected in all of the pcDNA3 HBsAg immunized mice, but none in control mice; serum levels of Th1 cytokines, interleukine 2, and interferon gamma, were significantly higher in pcDNA3 HBsAg immunized mice than these in controls. It is indicated that the good responses of cellular and humoral immunity can be induced by injection of pcDNA3 HBsAg in Balb/C mice. |
| |
| Keywords: | hepatitis B virus DNA gene vaccine mice |
| 本文献已被 CNKI 等数据库收录！ |
