| Establishment of Retinoblastoma Model in NOD-SCID Mice and Study of Metastasis |
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| 引用本文： | Bo Zhang,Yongping Li,Xiufeng Zhong,Wenge Huang,Li Nie,Wenxin Zhang Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510060,China. Establishment of Retinoblastoma Model in NOD-SCID Mice and Study of Metastasis[J]. 眼科学报, 2005, 21(3): 185-191 |
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| 作者姓名： | Bo Zhang Yongping Li Xiufeng Zhong Wenge Huang Li Nie Wenxin Zhang Zhongshan Ophthalmic Center Sun Yat-sen University Guangzhou 510060 China |
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| 作者单位： | Bo Zhang，Yongping Li，Xiufeng Zhong，Wenge Huang，Li Nie，Wenxin Zhang Zhongshan Ophthalmic Center，Sun Yat-sen University，Guangzhou 510060，China |
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| 基金项目： | This project was supported by a project grand of the Science and Technology of Guangdong Province (2003A3020302). |
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| 摘 要： | Purpose: To establish model of retinoblastoma subcutaneously in NOD-SCID mice and study rules of formation and distribution of retinoblastoma metastasis. Methods: Retinoblastoma cells SO-RB50 were inoculated subcutaneously in NOD-SCID mice. Animal acts and tumor formation, growth and metastasis in NOD-SCID mice were observed. Primary and metastatic tumors were studied pathohistologically by HE and immunohistochemical staining. Results: The latent periods of tumor growth were 12-19 days and the taken rate of tumor was 100%. 32 days later, 5 NOD-SCID mice were found with tumors that had metasta sized to areas mainly located in the abdominal cavity and the side of the kidney; the metastatic time of tumors in the mice also differed. The tumor cells of the primary nodules and the metastasis were similar with human retinoblastoma cells and positive in immunohistochemical staining of NSE. Conclusion: The subcutaneous model of retinoblastoma in NOD-SCID mice showed a high taken rate and a short latent period of tumor, which had a high metastatic rate and was the best model in research of behaviors of retinoblastoma at present. Eye Science 2005 ;21:185-191.
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| 关 键 词： | 眼癌 动物实验 NOD-SCID 小鼠 肿瘤转移 |
Establishment of Retinoblastoma Model in NOD-SCID Mice and Study of Metastasis |
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| Bo Zhang,Yongping Li,Xiufeng Zhong,Wenge Huang,Li Nie,Wenxin Zhang. Establishment of Retinoblastoma Model in NOD-SCID Mice and Study of Metastasis[J]. Eye science, 2005, 21(3): 185-191 |
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| Authors: | Bo Zhang Yongping Li Xiufeng Zhong Wenge Huang Li Nie Wenxin Zhang |
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| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060,China |
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| Abstract: | Purpose: To establish model of retinoblastoma subcutaneously in NOD-SCID mice and study rules of formation and distribution of retinoblastoma metastasis. Methods: Retinoblastoma cells SO-RB50 were inoculated subcutaneously in NOD-SCID mice. Animal acts and tumor formation, growth and metastasis in NOD-SCID mice were observed. Primary and metastatic tumors were studied pathohistologically by HE and immunohistochemical staining. Results: The latent periods of tumor growth were 12-19 days and the taken rate of tumor was 100%. 32 days later, 5 NOD-SCID mice were found with tumors that had metasta sized to areas mainly located in the abdominal cavity and the side of the kidney; the metastatic time of tumors in the mice also differed. The tumor cells of the primary nodules and the metastasis were similar with human retinoblastoma cells and positive in immunohistochemical staining of NSE. Conclusion: The subcutaneous model of retinoblastoma in NOD-SCID mice showed a high taken rate and a short latent period of tumor, which had a high metastatic rate and was the best model in research of behaviors of retinoblastoma at present. Eye Science 2005 ;21:185-191. |
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| Keywords: | NOD-SCID mice retinoblastoma metastasis animal model |
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