| 抗人红细胞抗体和HIV抗原融合蛋白的构建及活性鉴定 |
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| 引用本文： | 胡燕,杨健洋,朱雷,侯俊,沈宏辉,貌盼勇. 抗人红细胞抗体和HIV抗原融合蛋白的构建及活性鉴定[J]. 中华实验和临床病毒学杂志, 2007, 21(1): 76-78 |
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| 作者姓名： | 胡燕 杨健洋 朱雷 侯俊 沈宏辉 貌盼勇 |
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| 作者单位： | 100039,北京,解放军第三○二医院传染病研究所病毒研究室 |
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| 摘 要： | 目的构建及表达抗红细胞(RBC)单链抗体(ScFv)和HIV抗原的融合蛋白,并做活性测试。方法将鼠抗人红细胞单链抗体可变区重链、轻链基因与HIV抗原基因相连,重组于高效表达载体中,并在大肠埃希菌中表达。结果经ELISA检测和红细胞凝集检测,证明表达的融合蛋白具有HIV抗原和抗人红细胞的双重活性,可使含有HIV抗体的红细胞悬液凝集。结论融合蛋白构建成功,可用于HIV的快速检测。
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| 关 键 词： | 融合蛋白 HIV 凝集试验 |
| 收稿时间： | 2005-06-24 |
Expression,purification and characterization of an anti-hnman RBC ScFv-HIV gpl60 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood |
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| HU Yan,YANG Jian-yang,ZHU Lei,HOU Jun,SHEN Hong-hui,MAO Pan-yong. Expression,purification and characterization of an anti-hnman RBC ScFv-HIV gpl60 fusion protein for hemagglutination-based rapid detection of antibodies to HIV in whole blood[J]. Chinese journal of experimental and clinical virology, 2007, 21(1): 76-78 |
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| Authors: | HU Yan YANG Jian-yang ZHU Lei HOU Jun SHEN Hong-hui MAO Pan-yong |
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| Affiliation: | Department of Virology, The No. 302 Hospital of the People's Liberation Army, Beijing, 100039, China. |
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| Abstract: | Objective To construct and express anti-human RBC and HIVgp160 fusion protein for rapid detection of antibody to HIV.Medthod The gene of the anti human RBC ScFv and HIV antigen were constructed together into expression vector. The fusion protein was expressed in E.coli. Results The fusion protein was proved to be able to bind both anti-RBC and HIVgp160.It could cause agglutination of human RBC when HIVgp160 was present.Conclusions The fusion protein has the potential in rapid detection of HIV. |
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| Keywords: | Fusion protein HIV Agglutination test |
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