桂枝中桂皮醛、肉桂酸的含量与分布研究

 目的以桂皮醛和肉桂酸为指标,优化桂枝的提取工艺,并测定不同地区桂枝中桂皮醛及肉桂酸的含量,探讨桂枝皮部 与木质部的含量差异。方法 高效液相色谱法。色谱柱:Kromasil C₁₈(4．6 mm×250 mm,5μg);流动相:乙腈-0.1％磷酸水溶液 (43:57);检测波长:285 nm,温度:20℃,流速:1 mL·min^-1。结果 确定了桂枝提取的最佳工艺,即用甲醇50 mL,60℃热提40 min,可使桂皮醛和肉桂酸充分地被提取。结论测得广西平南县桂枝中桂皮醛的含量最高,广西防城市桂枝中肉桂酸的含量 最高。桂枝皮部中桂皮醛的含量较高,木质部中肉桂酸的含量较高。

Study on contents of cinnamaldehyde and cinnamic acid and distribution of Ramulus Cinnanomi

OBJECTIVE To optimize the extraction methods of Ramulus cinnamomi with cinnamaldehyde and cinnamic acid as reference, and evaluate the variety among samples harvest from different place and between bark and xylem parts of the plant.METHODS A HPLC method was performed on a Kromails C₁₈(4.6 mm×250 mm,5 μm)column. The mobile phase consisted of CH₃CN-H₂O (with 0.1%H₃PO₄) (43:57) with the detection wavelength at 285 nm. The column temperature was at 20 ℃ and the flow rate was 1.0 mL·min<>sup-1.RESULTS 200 mg Crude herb powder was added to 50 mL methanol under 60 ℃ for 40 min to obtain the highest content of cinnamaldehyde and cinnamic acid in the extract.CONCLUSION Samples from Pinnan,Guangxi province, own the highest content of cinnamaldehyde. While, samples from Fangcheng,Guangxi province,own the highest content of cinnamic acid.The bark contains more cinnamaldehyde and less cinnamic acid than xylem.