基质金属蛋白酶3在实验性大鼠腹主动脉瘤模型中的表达

目的：检测基质金属蛋白酶3（MMP－3）在大鼠正常动脉及腹动脉瘤模型组织中的表达，以探讨其在腹主动脉瘤发病机制中的作用。方法：建立大鼠腹主动脉瘤灌注模型，应用免疫组织化学和分子原位杂交技术检测MMP－3的表达。结果：正常动脉壁组织MMP－3蛋白表达为弱阳性或阴性，而动脉瘤组织中MMP－3蛋白表达显著增高，阳性率为8／8／MMP－3阳性染色主要位于中膜的平滑肌细胞和外膜的炎症细胞，其中炎症细胞浸润的区域染色明显增强，MMP－3 mRNA在动脉瘤组织中呈阳性表达，在动脉外膜炎症细胞浸润的区域和小血管聚集区呈强阳性；对照组动脉组织未见MMP－3 mRNA阳性表达。结论：腹主动脉瘤组织中MMP－3蛋白及mRNA较正常动脉组织表达显著增高，尤其在炎症细胞浸润的区域表达明显增强；提示MMP－3可能在腹主动脉瘤发生发展过程中起到关键的起始因子的作用。

Expression of matrix metalloproteinase-3 in experimental abdominal aortic aneurysm rat model

Objective:To investigate matrix metalloproteinase 3 (MMP 3) expression in experimental abdominal aortic aneurysms (AAA) rat model. Methods:The expression of MMP 3 were observed in 8 rat AAA models and 8 normal control aortic tissues using immunohistochemistry and molecular in situ hybridization technique. Results:The positive rate of MMP 3 protein expression was 8/8 in AAA and it was markedly increased compared with that of control samples( P <0.01). The positive expression of MMP 3 protein, mostly in adventitia inflammatory infiltration areas and media vascular smooth muscle cells in AAA model tissues, was much stronger than that of control aortic tissues. The expression of MMP 3 mRNA in AAA group was much stronger than that in control group. The expression pattern of MMP 3 mRNA in AAA was consistent with expression of MMP 3 protein. Conclusion:The protein and mRNA expression of MMP 3 is markedly increased in experimental AAA than in control abdominal aortic tissues. MMP 3 may be a key initial factor in experimental AAA formation and progression.