复方牡蛎根管充填糊剂的体外细胞毒性研究

目的:评价根管充填材料复方牡蛎糊剂的细胞毒性。方法:将根管充填材料的粉剂及粉液固化物分别制备成不同含量(50mg/ml、100mg/ml、200mg/ml)的浸提液作为试验组,RPMI1640培养液作为阴性对照组,应用V79细胞作为实验细胞;各组分别在细胞传代后培养的第2、4、7天时间段以倒置显微镜观察V79细胞的形态及增殖情况,并用MTT法测得各组在各个时间段的吸光度值,应用SPSS12.0软件包对数据进行统计学分析,评价材料的细胞毒性。结果:各实验组在细胞传代后培养的第2、4、7天,镜下观察细胞数量逐渐增多,呈多角形,汇集成群,与阴性对照组无显著差异;各实验组的吸光度值在各个时间段与阴性对照组均无显著性差异(P>0.05),且随培养时间的延长,吸光度值增大。结论:实验显示,复方牡蛎根管充填糊剂无细胞毒性。

Cytotoxicity study of composite oyster shell paste as a root canal filling material

PURPOSE: To evaluate the cytotoxicity of new root canal filling material-composite oyster shell paste.METHODS: According to the evaluated criteria of medical materials, biology and animal trials recommended in ISO 10993,the extracts of powder part and the mixture of the new material were prepared separately and selected as the experimental. The RPMI1640 subject was used as the negative control and the V79 cell as the experimental cell. The cells were cultured in extract of different thickness compound for 2,4,7 days respectively and the changes of the cells were observed under microscope.The proliferation of the cells were examined and the optical density(OD)was obtained using MTT assay. SPSS12.0 software was used for statistical analyses. RESULTS: During all culture periods,the V79 cells showed normal morphology and proliferation increased with culture time. The OD of the experimental groups were not significantly different from that of the negative groups(P>0.05)and the OD increased as time goes on. CONCLUSION: The new root canal filling material shows no detected cytotoxic effect from this study.