TaqMan real-time PCR for detection and quantitation of squash leaf curl virus in cucurbits |
| |
| Authors: | Kuan Cheng-Ping Huang Hung-Chang Chang Chia-Che Lu Yi-Lin |
| |
| Affiliation: | a Division of Biotechnology, Taiwan Agricultural Research Institute, Wufeng, Taichung, Taiwan
b Institute of Biomedical Sciences, National Chung-Hsing University, Taichung 40227, Taiwan |
| |
| Abstract: | A real-time PCR assay based on the TaqMan chemistry was developed for reliable detection and quantitation of the squash leaf curl virus (SLCV) in melon and squash plants. This method was highly specific to SLCV and it was about one thousand times more sensitive than the conventional PCR method. The protocol of the real-time PCR established in this study enabled detection of as little as 102 copies of SLCV DNA with CP gene as the target. This TaqMan real-time PCR assay for detection and quantitation of SLCV would be a useful tool for application in quarantine and certification of SLCV in cucurbits as well as in the research of disease resistance and epidemiology. |
| |
| Keywords: | Squash leaf curl virus Squash Cucurbita pepo Melon Cucumis melo Real-time PCR |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
