不同剂量吡喹酮治疗曼氏裂头蚴感染小鼠的疗效观察

目的观察不同剂量吡喹酮对曼氏裂头蚴感染小鼠的疗效。方法156只昆明小鼠分2批感染,每只经口(灌胃)接种感染5条裂头蚴。第1批36只小鼠分为6组(每组6只),1-5组小鼠分别接种含不同浓度吡喹酮培养液中培养3d的裂头蚴,6组小鼠接种正常培养液中培养3d的裂头蚴,作为对照组;第2批120只小鼠分成12组(每组10只),分别接种青蛙或蝌蚪体内的裂头蚴,1-9组于感染后1周或5周用不同剂量的吡喹酮灌胃治疗,10-12组为对照组。各组小鼠于治疗结束后1周或2周剖杀,收集裂头蚴数并计算各组的平均检出虫数和减虫率。结果第1批小鼠接种在10-40μg/ml吡喹酮中培养3d的裂头蚴后,检出虫数与对照组相比差异无统计学意义(P0.05),接种在50μg/ml吡喹酮中培养3d的裂头蚴后,减虫率仅为16.60%,各剂量组减虫率之间差异无统计学意义(P0.05)。第2批小鼠感染青蛙体内裂头蚴1周后,用200、400、800mg/kg吡喹酮治疗后1周和2周的检出虫数与对照组相比,差异均无统计学意义(P均0.05),相同剂量组治疗后1周和2周后减虫率差异均无统计学意义(P均0.05);感染蝌蚪体内裂头蚴1周后,200、400mg/kg吡喹酮治疗后1周检出虫数与对照组相比差异均无统计学意义(P均0.05),800mg/kg治疗后1周减虫率为17.02%,各剂量组减虫率之间差异无统计学意义(P0.05)。小鼠感染青蛙体内裂头蚴5周后,用1200、1800mg/kg吡喹酮治疗1周和2周后,检出虫数差异无统计学意义(P0.05),但均明显高于对照组(P均0.05),相同剂量组减虫率之间的差异无统计学意义(P0.05)。结论吡喹酮(10～50μg/ml)在体外对裂头蚴无明显杀伤作用,但大剂量(1800mg/kg)灌胃时对裂头蚴感染小鼠疗效较好。

Therapeutic effect of different dosages of praziquantel on mice infected with Sparganum mansoni

Objective To observethe efficacy of mice infected with Sparganum mansoni by using different dosages of praziquantel.Methods A total of 156 Kunming mice were divided into 2 batches.each of them wag orally infted with 5 spargana.Thirty-six mice in the first batch were equally divided into 6 groups.the mice in group 1-5 were inoculated with spargana cultured in different concentrations of praziquantel for 3 days,the group 6 served as a control.One hundred and twenty mice in the second batch were equally divided into 12 groups,each mouse was inoculated with spargana obtained from frogs or tadpoles,group 1-9 were treated by different desages of praziquantel 1 or5 weeks post infection.group 10-12 served as controls.All of the mice wore sacriftced and dissectedl or 2 weeks after the treatment.the mean number of worms recovered was cmculated and worm reduction rates were determined.Results The number of worm recovered from mice infected with spargana cultured in 10-40 μg/ml of praziquantel had no significant difference with that of the control(P>0.05).The worm reduction rate wag 16.60%while the spargana beins cultured in 50 μg/ml of praziquantel.The worm reduction rates of the mice that sacrificed 1 week or 2 weeks after being treated by the same dosage of praziquantel had no significant difference(P>0.05).When being treated with 200.400 or 800 mg/kg of praziquantel 1 week post infection,the number of worm recovered from mice infected with spargana from frogs had no significant difference with those of the control 1 and 2 weeks after the treatment(P>0.05).The worm reduction rates between the groups with the same dosage 1 week and 2 weeks post treatment had no significant difference(P>0.05).When being treated with 200 or 400 mg/kg of praziquantel 1 week post infection,the number of worm recovered from mice infected with spargana from tadpoles had no statistically difference with that of the control 1 week after treatment (P>0. 05). The worm reduction rate of mice was only 17.02% while being treated with 800 mg/kg of praziquantel. The worm reduction rates among groups with different dosages had no significant difference (P>0.05). Compared with the mice infected with spargana from frogs treated with 1 200 or 1 800 mg/kg of praziquantel 5 weeks post infection, the difference between the numbers of worm recovered from mice 1 week and 2 weeks after treatment had no statistically significance (P > 0.05), but they were significantly higher than those of the controls (P<0.05 ). The worm reduction rates among the groups with the same dosage had no significant difference (P>0.05). Conclusions Praziquantel (10-50 μg/ml) has no evident killing effect on spargnna in vitro, but when the dosage is higher(1 800 mg/kg), it has certain efficacy for treating the mice infected with spargana by oral inoculation.