不同梅毒血清学诊断方法的临床检测与效果评价

目的探讨rTpN15-17-47-ELISA、RPR和TPPA三种用于梅毒血清抗体检测方法的敏感性和特异性。方法三种方法分别检测50份无梅毒病史健康人血清、11例类风湿关节炎（RA）、5例肾病综合征（NS）及122例确诊梅毒患者血清标本中梅毒抗体，对各方法的检测结果进行比较。结果rTpN15-17-47-ELISA对所有健康人血清、RA患者及NS患者血清检测结果均为阴性，对梅毒患者血清标本检测阳性率为97．5％，与TPPA（96．7％）相似（P〉0．05），且均高于RPR（80．3％）。结论本研究中建立的以重组融合蛋白质rTpN15-17-47为抗原的ELISA有望成为高度敏感和特异的梅毒患者血清学筛查方法。

Clinical Evaluation of Different Serological Methods for Diagnosis of Treponema Pallidum

Objective To evaluate the sensitivity and specificity of rTpN15-17-47-ELISA, RPR and TPPA methods in serological diagnosis of syphilis. Methods The serum samples from 50 healthy individuals, 11 RA patients,5 NS patients and 122 syphilis patients were detected by using rTpN15-17-47-ELISA, RPR and TPPA. The detection effect of the ELISA was compared to those of RPR and TPPA. Results The detection results of rTpN15-17-47-ELISA was negative for the serum samples from healthy individuals, RA and NS patients, while presented 97.5% positive detection rates for the serum samples from syphilis patients. The positive detection rates of rTpN15-17-47-ELISA was similar to that of TPPA （ 96.7% ） （P 〉 0.05 ）, both the positive rates of rTpN15-17-47-ELISA and TPPA were significantly higher than that of RPR （ 80.3% ）. Conclusion rTpN15-17-47-ELISA established in this study is great hopeful to become sensitivity and specificity methods for serological screening and detection of syphilis.