雷帕霉素对乳腺癌多药耐药细胞的耐药逆转作用初探

目的探讨雷帕霉素对乳腺癌多药耐药细胞系MCF-7／ADR的耐药逆转作用。方法MTT法测定10μM雷帕霉素、阿霉素（10、50、100、500、1000、5000、20000、40000、100000ng／mL）、雷帕霉素联合阿霉素对MCF-7和MCF-7／ADR细胞的增殖率的影响。结果对于MCF-7细胞的增殖率，10μM雷帕霉素对其无显著影响；500ng／mL及以上浓度的阿霉素显著降低增殖率；联合10μM雷帕霉素后，能降低增殖率的浓度仍是500ng／mL及以上。对于MCF-7／ADR细胞，10μM雷帕霉素对其增殖率无显著影响；40000ng／mL及以上浓度的阿霉素降低其增殖率；联合10μM雷帕霉素后，1000ng／mL及以上浓度的阿霉素能降低其增殖率。结论雷帕霉素对MCF-7／ADR细胞有耐药逆转作用。

The Initial Exploration about Reverse Effect of Rapamycin on Multidrug Resistance of Human Breast Cancer Cell

Objective To explore the reverse effect of rapamycin on multidrug resistance of human breast cancer cell MCF-7/ADR. Methods MTT was applied for detecting the proliferation rates of MCF-7 cells and MCF-7/ADR cells which treated by rapamycin （ 10μ M ）, ADM （ 10,50,100,500, 1000,5000,20000,40000, 100000ng/mL ）, or rapamycin combined with ADM. Results For the proliferation rate of MCF-7 cells, rapamycin （ 10 μM ） did not affect it, ADM （ 500, 1000,5000,20000,40000,100000 ng/mL ） reduced it, and combined with rapamycin, the concentration of ADM which could reduce it was greater than or equal to 500 ng/mL. For the proliferation rates of MCF-7/ADR, rapamycin （ 10 W M ） did not affect it, ADM （ 40000,100000ng/mL ） reduced it, and combined with rapamycin, the concentration of ADM which could reduce it was greater than or equal to 1000ng/mL. Conclusion Rapamycin could reverse the muhidrug resistance of MCF-7 cells.