|
|||||
|
|
| 体外培养对骨髓间充质干细胞凋亡的影响 | |
| 引用本文： | 董海,陆骅,汤亭亭,周之德,陈晓东,史桂英. 体外培养对骨髓间充质干细胞凋亡的影响[J]. 中国矫形外科杂志, 2004, 12(9): 686-688 |
| 作者姓名： | 董海 陆骅 汤亭亭 周之德 陈晓东 史桂英 |
| 作者单位： | 1. 上海第二医科大学附属新华医院骨科,上海市控江路1665号,200092 2. 上海第二医科大学附属第九人民医院骨科实验室 3. 上海第二医科大学细胞生物研究室 |
| 摘 要： | 目的 :研究成人骨髓间充质干细胞在体外培养条件下 ,传代次数与细胞凋亡的关系。方法 :采用An nexinV/PI双染色法 ,在流式细胞仪上检测不同传代数的成人骨髓间充质干细胞的凋亡情况。结果 :传代次数越多 ,骨髓间充质干细胞的凋亡率及死亡率越高 ,存活的正常细胞越少。结论 :第 4代以内的细胞增殖能力强 ,凋亡率低 ,适宜于组织工程研究 |
| 关 键 词： | 间充质干细胞 骨髓 细胞培养 细胞凋亡 传代培养 增殖 组织工程 |
| 文章编号： | 1005-8478(2004)09-0686-03 |
| 修稿时间： | 2003-11-17 |
Apoptosis of adult mesenchymal stem cells (MSCs) cultured in vitro |
|
| DONG Hai,LU Hua,TANG Ting ting,et al. Apoptosis of adult mesenchymal stem cells (MSCs) cultured in vitro[J]. The Orthopedic Journal of China, 2004, 12(9): 686-688 | |
| Authors: | DONG Hai LU Hua TANG Ting ting et al |
| Affiliation: | DONG Hai,LU Hua,TANG Ting ting,et al Department of Orthopaedics,Xinghua Hospital,Shanghai the Second Medical University,Shanghai 200092 |
| Abstract: | Objective:To investigate the relationship between apoptosis rate of the adult MSCs cultured in vitro with the times of subculture passage Method:The apoptosis rate of adult MSCs within different passages was investigated by flowcytometry with Annexin V/PI Result:The more times MSCs was subcultured,the rate of apoptosis and death was higher and the fewer alived Conclusion:The MSCs within 4 passages should be used as the seeding cells of tissue engineering because of strong proliferative ability and low rate of apoptosis |
| Keywords: | Mesenchymal stem cells Bone marrow Cell culture Apoptosis Subculture Proliferation Tissue engineering |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |