Stat3和Stat5在分化型甲状腺癌组织中的表达及意义

目的:研究分化型甲状腺癌(DTC)组织中信号转导和转录激活因子Stat3和Stat5的表达情况及其与甲状腺癌临床病理因素和相关抑癌基因之间的关系，探讨Stat3和Stat5在DTC发生、发展中的作用。方法：应用免疫组织化学 SABC法检测42例DTC［包括38例乳头状癌(PTC)和4例滤泡状癌(FTC)］、30例甲状腺腺瘤（TA）及15例正常甲状腺组织(NTT)中Stat3、Stat5、P53及P21蛋白的表达，比较不同组织中阳性细胞表达率,分析其与DTC临床病理参数的相关性。结果：Stat3和Stat5在DTC组织中的阳性率显著高于TA和NTT(P<0.05) ，二者表达率与DTC病理分型密切相关（r=0.76,P<0.05；r=0.80，P<0.05）；Stat3和Stat5在PTC中的表达率明显低于FTC(P<0.05)，且Stat5 的表达率明显高于Stat3，二者呈正相关（r=0.78，P<0.05）。DTC组织中P53蛋白阳性表达率（30.9%）明显高于TA(0%)和NTT（0%）(P<0.05) 。P21蛋白阳性表达率（14.3%）在DTC组织中明显降低。Stat3和Stat5在DTC组织中表达率分别为54.8%和66.7%，明显高于P53表达率（30.9%），且分别与P53表达率呈正相关(r=0.77,P<0.05；r=0.72，P<0.05)，与P21表达率呈负相关（r=－0.87,P<0.05；r=－0.67,P<0.05）。结论：Stat3和Stat5参与DTC病理分型，其异常表达率可能与p53基因的突变、抑癌基因p21的失活有关。Stat3和Stat5表达率的异常增高可能是DTC发生、发展的原因之一。

Expressions of Stat3 and Stat5 in differentiated thyroid carcinoma tissues and significances

Objective To investigate the expressions of Stat3 and Stat5 in differentiated thyroid carcinoma(DTC)tissues and their relationships with clininopathological factors,and study the roles of Stat3 and Stat5 in the occurrence and development of DTC.Methods The expressions of Stat3,Stat5,P53,P21 in 42 patients with DTC including 38 papillary thyroid carcinoma(PTC)and 4 follicular thyroid carcinoma(FTC),30 cases of thyroid adenomas(TA)and 15 normal thyroid tissues(NTT)adjacent to adenomas were detected by immunohistochemical(SABC)staining method.The positive expression rates in different tissues were compared,and their corelations with clinicopathological factors of DTC were analyzed.Results The positive expression rates of Stat3 and Stat5 in DTC were significantly increased compared with TA and NTT respectively(P<0.05),their expression rates were closely correlated with pathological typing of DTC(r=0.76,P<0.05;r=0.80,P<0.05);the expression rates of Stats and Stat5 in PTC were lower than those in FTC(P<0.05).The expression rate of Stat5 was significantly higher than that of Stat3 and they had positive correlation(r=0.78,P<0.05).The positive expression rate of P53 in DTC tissue(30.9%)was higher than those in TA(0%)and NTT(0%)(P<0.05).The positive expression rate of P21 protein in DTC tissue was significantly decreased.The expression rates of Stat3(54.8%)and Stat5(66.7%)were significantly higher than that of P53(30.9%)and there were positive correlations with P53(r=0.77,P<0.05;r=0.72,P<0.05)and negative correlation with P21(r=-0.87,P<0.05;r=-0.67,P<0.05).Conclusion The abnormal expressions of Stat3 and Stat5 may play important roles in the carcinogenesis of thyroid carcinoma.Perhaps the abnormal expressions of Stat3 and Stat5 may be caused by mutation of p53 and deactivation of p21.The abnormal increase of the expression rates of Stat5 and Stat3 may lead to the occurrence and development of DTC.