Binding of muscarine receptor antagonists to pig coronary smooth muscle |
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| Authors: | I Rinner H N Doods K J van Charldorp D Davidesko P A van Zwieten |
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| Institution: | (1) Department of Pharmacology, Academic Medical Centre and Academic Hospital, University of Amsterdam, NL-1018 TV Amsterdam, The Netherlands;(2) Institute of Functional Pathology, University of Graz, Mozartgasse 14, A-8010 Graz, Austria |
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| Abstract: | Summary In order to characterize the muscarinic binding site on coronary smooth muscle, we investigated the binding properties of (3H)quinuclidinyl benzilate (QNB) in membrane preparations of pig coronary arteries and atria. Scatchard analysis and Hill plot showed that (3H)QNB binds to a single population of sites in both tissues. The binding profiles of the muscarine receptor antagonists atropine, 11-((2-((dimethylamino)methyl)-1-piperidinyl)acetyl)-5,11-dihydro-6H-pyrido(2,3-b) (1,4)benzodiazepine-6-one (AF-DX 116), pirenzepine, and 4-diphenylacetoxy-Nmethylpiperidine methiobromide (4-DAMP) in both tissues were compared with binding data from other tissues, representative for different muscarinic binding site subtypes. It is concluded that the pig coronary smooth muscle muscarinic binding site is different from M1 and M2 binding sites investigated so far.
Send offprint requests to I. Rinner |
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| Keywords: | Pig coronary arteries Pig atria Muscarine receptor Muscarine receptor antagonists |
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