银杏苦内酯B对重症急性胰腺炎胰腺组织病理变化的影响

目的 通过银杏苦内酯B(BN52021)对血小板活化因子(PAF)的拮抗作用,探讨BN52021治疗重症急性胰腺炎(SAP)的疗效.方法 Wistar大鼠180只按随机数字表法分为对照组(60只)、SAP组(60只)、BN52021组(60只),每组按不同时相点(1、2、3、6、12、24 h)分为6小组,分别监测血清淀粉酶的变化,用RT-PCR和Western blot法分别监测胰腺组织PAF受体mRNA和蛋白表达的变化,同时对胰腺组织进行病理学观察.多组比较采用单因素方差分析.结果 血清淀粉酶和病理学结果显示SAP制模成功,BN52021组血清淀粉酶在3、6、24 h(4185 ±148)U/L,(3785 ±124)U/L,(1360 ±161)U/L]较SAP组(4799 ±107)U/L,(4920±140)U/L,(2283±127)U/L]显著降低,病理学评分在3、6、12 h(5.95 ±0.19,5.55 ±0.36,6.72 ±0.30)较SAP组(8.85±0.39,9.15±0.55,10.10±0.65)显著降低;PAF受体mRNA及蛋白检测结果显示,SAP组和BN52021组早期逐渐上升(0.49±0.09～0.71±0.14,0.43±0.06～1.69±0.06),在3 h达到高峰,3组比较差异有统计学意义(F=4.58.6.24,P<0.05).结论 PAF受体在SAP大鼠胰腺组织中表达是动态变化的,PAF受体参与了SAP病情的发生、发展;BN52021可降低SAP血清淀粉酶和改善胰腺组织病理变化,发挥了一定的治疗作用,但对胰腺组织PAF受体的表达无显著影响.

Effects of ginkgolide B (BN52021) on pathological changes Of pancreatic tissue in rats with severe acute pancreatitis

Objective To investigate the inhibitory effect of ginkgolide B (BN52021) on severe acute pancreatitis (SAP) via detecting the antagonistic effect of BN52021 on platelet-activating factor (PAF). Methods One hundred and eighty Wistar rats were randomly divided into control group (n = 60), SAP group (n = 60) and BN52021 group (n =60) according to the random number table. The 3 groups were divided into 6 subgroups at different time points after operation (1 h, 2 h, 3 h, 6 h, 12 h, and 24 h). The changes of serum amylase in each group were monitored. The expression of platelet-activating factor receptor (PAFR) mRNA and protein was detected by RT-PCR and Western blot, and the pathological changes of pancreatic tissues were observed. All the data were analyzed by one-way ANOVA. Results Serum amylase level and pathological results showed that it was successful in preparing SAP model. The serum amylase levels at postoperative hour 3, 6 and 24 were (4185 ±148) U/L, (3785 ± 124) U/L and (1360 ± 161) U/L in BN52021 group, which were significantly lower than those in SAP group (4799 ± 107) U/L, (4920 ± 140) U/L, (2283 ± 127) U/L)]. The pathological scores at postoperative hour 3, 6, 12 were 5.95±0. 19, 5.55±0.36, 6.72±0. 30 in BN52021 group, which were significantly lower than those in SAP group (8.85 ± 0.39, 9.15 ± 0.55, 10.10 ±0. 65). The mRNA and protein expression of PAFR were gradually increased at the early stage (0.49 ± 0.09-0.71 ± 0.14 vs 0. 43 ～ O. 06-1.69 ± 0.06), and reached peak at postoperative hour 3. The expression levels of PAFR mRNA and protein in BN52021 group and SAP group at postoperative hour 3 had statistical difference among the 3 groups (F = 4.58, 6.24, P < 0.05). Conclusions The expression of PAFR mRNA and protein in the pancreatic tissue of SAP rats is dynamically changing. PAFR plays an important role in the occurrence and progression of SAP. BN52021 can reduce the expression of serum amylase and improve the pancreatic pathological changes, but it has no effect on the expression of PAFR in pancreatic tissue.