苦木中生物碱测定方法的研究

本文报道了在高效硅胶薄层上用氯仿(水饱和)—甲醇(40:0.5)展开系统,分离了苦木中三种咔巴林生物碱(Ⅰ,Ⅲ,Ⅳ)及一种铁屎米酮生物碱(Ⅱ)。用甲酸处理后,薄层扫描法测定。本法决速、灵敏,检出限度可达1×10^-8g,各生物碱的线性范围为0.05～0.3μg,并用此法测定了十一个不同地区及不同部位的苦木生药中的生物碱含量。

STUDIES ON THE DETERMINATION OF ALKALOIDS IN PICRASMA QUASSIOIDES (D.DON) BENN.

A simple, sensitive and accurate method for separation and determination of the alkaloids, 1-formyl-β-carboline (Ⅰ),. 4, 5-dimethoxycanthin-6-one (Ⅱ), 1-vinyl-4, 8-dimethoxy-β-earboline (Ⅲ) and 1-formyl-4-methoxy-β-carboline (Ⅳ), in Ku-Mu Picrasma quassioides (D. Don) Benn.] is described.Sample solution was applied at a point 1 cm from the bottom edge of the HPTLC silica gel plate (10 × 10cm). Chloroform (water saturated)—methanol (40:0.5) was used as the developing solvent. The plate was saturated for 30 min and then developed twice for 9 cm using ascending technique. The plate was fumigated with formic acid for 1 h at room temperature to intensify the spot color. The spots were scanned with a Sehimadzu CS-910 TLC scanner. The content of four alkaloids in Ku-Mu was calculated by comparison with standards spotted on the same plate.The standard curve was linear in the range oft the 0.05～0.30 μg for the four alkaloids. The method has been applied to the analysis of different samples. This method can be used for the quality control of Ku-Mu preparations for clinical use.