精浆弹性蛋白酶水平与精液主要参数和精子功能的相关性分析

目的:探讨精浆弹性蛋白酶与精液主要参数和指标的关系。方法:用酶联免疫吸附法(ELISA)检测精浆中的弹性蛋白酶,按照WHO人类精液实验室手册要求进行精液常规分析、精子形态分析,检测精子顶体酶活性、精浆抗体(AsAb)、解脲支原体等,分析弹性蛋白酶与男性不育相关因素的关系。结果:209例男性不育患者中,43例患者精浆弹性蛋白酶≥290ng/ml,设为炎症组;166例患者精浆弹性蛋白酶<290ng/ml,设为非炎症组。炎症组的精子密度、精子活动率、a+b级活力精子率、精子顶体酶阳性率均低于非炎症组(P<0.05);而精子畸形率、精浆抗体(AsAb)、解脲支原体阳性率均高于非炎症组(P<0.05)。两组的精液量、PH值和液化时间差异无统计学意义。结论:精浆弹性蛋白酶水平与精液质量有密切的关系,生殖道感染是导致男性不育的重要原因。

Correlation of seminal plasma elastase and semen major parameters and sperm function

Objective:To study the relationship between seminal plasma elastase and semen major parameters.Methods:The level of seminal plasma elastase was detected by ELISA(Enzyme-linked immunosorbent assay).Semen major parameters,abnormal morphological sperm rate,sperm acrosin reaction,AsAb and UU infection were measured and analyzed in 209 infertile men according to WHO laboratory handbook about human semen.Results:43 cases were men whose level of seminal plasma elastase was equal to or higher than 290 ng/ml in 209 male infertility,set up into inflammation group.The others(<290 ng/ml) were 166 cases,set up into non-inflammation group.Semen concentration,motility,a+b grade motility,and sperm acrosin reaction rate in inflammation group were lower than in non-inflammation group(P<0.05).Higher abnormal morphological sperm,UU infection rate and AsAb positive rate were observed in inflammation group(P<0.05).Sperm volume,PH value and liquefying time were not significantly different between inflammation and non-inflammation group(P>0.05).Conclusions:The level of seminal plasma elastase is associated with sperm quality,which can be considered as one of the important reasons resulting in male infertility.