Diversity of Human Anti-D Monoclonal Antibodies Revealed by Reactions with Chimpanzee Red Blood Cells |
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| Authors: | A. Blancher W. W. Socha and J. Ruffie |
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| Affiliation: | Centre Régional de Transfusion Sanguine, CHU Purpan, Toulouse, France;Laboratory for Experimental Medicine and Surgery in Primates, New York University Medical Center, New York, N.Y., USA;Laboratoire d'Anthrophologie Physique du Collège de France, Paris, France |
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| Abstract: | Fifty-three human anti-D monoclonal antibodies (mAbs) revealed a striking diversity of reactions in tests with panels of chimpanzee red blood cells (RBCs) of various R-C-E-F blood group phenotypes (counterparts of the human Rh-Hr groups). The reactivities of these antibodies, which depended on the agglutination technique used, could be classified into four main types. These patterns of reactivity of anti-D mAbs with chimpanzee RBCs showed only limited correlation with types of reactions observed with human D variant RBCs. Primate red cells may, therefore, constitute an independent test system for subclassification of human monoclonal antibodies. Comparison of reactivities of human anti-D mAbs with chimpanzee and human D variant RBCs confirms the homology between the chimpanzee Rc, and the human D antigens. The chimpanzee Rc shares with human D the epitopes epD5, epD6/7 and epD8, but lacks epitopes epD1, epD2, epD3 and epD4 of the Rh mosaic, thus resembling the human D variants IVb and Vc. |
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