| 布鲁氏菌强毒感染和菌苗免疫绵羊的鉴别诊断实验研究 |
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| 引用本文： | 阎守敦 李曼芳. 布鲁氏菌强毒感染和菌苗免疫绵羊的鉴别诊断实验研究[J]. 中国人兽共患病杂志, 1989, 5(5): 2-5 |
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| 作者姓名： | 阎守敦 李曼芳 |
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| 作者单位： | 新疆兽医防疫总站(阎守敦，张春生，阎晶华，徐敏)，中国预防医科院流行病学微生物学研究所(李爱芳)，伊犁地区畜牧兽医草原总站(胡守颜)，新疆八一农学院(贝念湘) |
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| 摘 要： | 本文报道用多种诊断方法进行绵羊布鲁氏菌病人工强毒(544A和羊Ⅱ)感染和菌苗(M_5和Rev-1)免疫鉴别诊断的实验研究。结果表明:改良ELISA、聚丙烯酰胺凝胶电泳(圆盘电泳)及查抗原的“BA-免疫斑点”试验,基本能将感染和免疫的绵羊区分开来,而其它标准试管凝集反应、微量补体结合反应、L-半胱氨酸凝集试验、离子交换法等对鉴别强毒感染和菌苗免疫只有不同程度的参考价值或区别不开。
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| 关 键 词： | 强毒感染 菌苗免疫 酶联免疫吸附试验 离子交换法 |
A preliminary Study on Methods for Different Diagnosis of Experimental Brucellosis Infected with Virulent Brucella |
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| Yan Shoudong. A preliminary Study on Methods for Different Diagnosis of Experimental Brucellosis Infected with Virulent Brucella[J]. Chinese Journal of Zoonoses, 1989, 5(5): 2-5 |
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| Authors: | Yan Shoudong |
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| Affiliation: | Yan Shoudong |
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| Abstract: | In this paper the methods for different diagnosis for sheep brucellosis infected with 554A and sheep 2 and immunized by inoculation of brucella vaccine (Rev-land MS) were described. The results showed that the modified ELISA, polyacry-lamide gelelectrophoresis (DISC)and BA-dot immanity assay can distinguish sheep: The specificity and sensitivity of these methods were higher then that of other conventional tests such as agglutination test, CF,ion-exchange chrimatography. |
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| Keywords: | Infection with virulent brucella Vaccination ELISA Ion-exchane chromatography. |
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