肾癌中CD133＋细胞和CD133-细胞生物学特性差异的研究

目的：研究人肾癌细胞系786-O和OS-RC-2中CD133＋细胞和CD133-细胞的生物学特性差异。方法：应用流式细胞仪检测肾癌细胞系786-O和OS-RC-2中CD133的膜表达状况；以免疫磁珠法将CD133＋细胞和CD133-细胞分离纯化，比较两者在光镜下的形态、生长特点、体外增殖能力、长期分化能力及体外克隆形成率的差异；流式细胞术分析两者细胞周期分布的变化，采用MTT法分析两者对化疗药物顺铂（DDP）（10、20、50、80／Lg／ml）敏感性的差别。结果：786-O和0S-RC-2均有CD133的少量表达，表达率为（8．12±0．86）％、（6．83±0．20）％，CD133＋细胞和CD133-细胞相比具有较强的体外增殖、克隆形成及长期分化能力；细胞耐药性实验表明CD133＋细胞、CD133-细胞对50bcg／m1DDP敏感性差异有统计学意义（P〈0．05）。结论：肾癌细胞系中CD133＋细胞具有肾癌干细胞的部分特征，能否作为肾癌干细胞的表面标志还需要进一步的实验加以明确。

Study on the Different Biological Characteristics of CD133+ Cells and CD133-Cells in Renal Cell Carcinoma

Objective： To investigate the different biological characteristics of CD133＋ cells and CD133- cells in human renal carcinoma cell line 786 - O and OS - RC - 2. Methods：The CD133 surface expression of both 786- O and OS-RC-2 cell lines was evaluated by flow cytometry. CD133＋ cells were isolated by MACS（magnetic activated cell sorting） and examimed in modality, vitro proliferation, growth characteristics and colony formation efficiency （ CFE ） . Flow cytometry was used to evaluate the cell cycle distribution and long-term differentiation ability. The sensitivity to diamminedichloroplatinum （DDP） （10.20, 50.80 μg/ml）between CD133＋ cells and CD133- cells were contrasted by drug sensitivity testing in vitro （MTT assay）. Results： A little CD133 expression were found in 786-0 and OS-RC-2 cell lines, the rate of expression were （8 . 12 ±0 . 86）%, （6 . 83 ± 0 . 20） %. It showed that isolated CD133＋ cells from 786-0 and OS-RC-2 cell lines are more proliferetive and have higher colony-forming and more long-term differentiation abilities than CD133- cells in vitro. Cell resistance experiments show that the difference of sensitivity to diamminedichloroplatinum （DDP） between CD133＋ cells and CD133 cells in 50μg/ml was significant （P〈0.05）. Conclusions：CD133＋ cells in RCC cell lines have some characteristics of cancer stem cells, but it is need further experiments to clarify whether CD133＋ cells can be the markers for the detection of cancer stem cells.