高效液相色谱-示差折光检测法同时测定维生素C葡萄糖注射液中维生素C与葡萄糖的含量

目的：建立高效液相色谱-示差折光检测法测定维生素C葡萄糖注射液含量的方法。方法：采用示差折光检测器色谱柱采用氨基键合硅胶色谱柱（4．6mm×250mm，5μm）；流动相为乙腈-0．02mol·L～KH2PO4溶液（pH6．0）（75：25）；柱温30℃，流速为1．0mL·min^-1。结果：维生素C和葡萄糖分别在0．10～1．00和0．25～2．50g·L^-1的浓度范围内有良好的线性关系（r=0．9997）；（r=0．9998），两者的平均回收率分别为100．2％与99．8％，RSD分别为1．5％与1．0％。结论：此法简便、快速、准确，适用于维生素C葡萄糖注射液的含量测定。

Simultaneous Assay of Vitamin C and Glucose in Vitamin C and Glucose Injection by HPLC with RID

Objective：To establish a method using HPLC - RID for simultaneous assay of Vitamin C and Glucose in Vitamin C and Glucose Injection. Methods：The detector was differential refraction detector. The Kromasil 100-5 NH2 （4. 6 mm × 250 mm,5 μm）set at 30℃ was used. The mobile phase was composed of acetonitrile-0. 02 mol · L^-1 KH2PO4 solution （ pH 6.0）. Results：The linear range of Vitamin C was 0. 10 - 1.00 g · L^-1 （r =0. 999 7） ,and Glucose was 0. 25 g · L^-2. 50 g · L^- （r =0. 999 7）. The average recovery of Vitamin C was 100. 2% （ RSD 1.5 % ）, and the average recovery of Glucose was 99. 8 % （ RSD 1.0 % ）. Conclusions ： This method is efficient, accurate and suitable for the simultaneous assay of Vitamin C and Glucose in Vitamin C and Glucose Injection.