骨水泥中抗癌药的释放、活性及抗肿瘤效应的实验研究

目的:确定化疗药丝裂霉素、长春新碱和5F-尿嘧啶掺入骨水泥后的释放及活性,评价其掺入骨水泥后经皮介入治疗小鼠移植瘤模型的抗肿瘤效应。材料和方法:采用洗提法和MTT法测量药物的释放及活性。60只昆明种小鼠,每只小鼠皮下注射,S180肉瘤细胞1×106,7天后随机分为4组,每组15只。3组为治疗组,每一组各以上述一种抗癌药骨水泥0.5ml瘤块内注入;另1组为对照组,仅注入单纯骨水泥0.5ml。记录每组10只荷瘤小鼠肿瘤大小变化,余5只作病理检查。结果:三种药物均可从骨水泥中释放并具有杀伤肿瘤细胞效应;3组治疗组中,肿瘤体积在1～10天内逐日缩小或不增大;而对照组中肿瘤体积持续增大。病理检查见治疗组水泥块周边肿瘤组织片状坏死。结论:骨水泥携带常用抗癌药具有抗肿瘤效应。

The Release,Activity and Anti-cancer Effect of Chemonlerapy Agents from Bone Cement

Purpose:To determine the release of mitomycin C,vincristine sulfate and 5-flauouracil mixed with bone cement and the anti-cancer activity of the release drugs.The anti-cancer effects of them by percutaneous interventional technique were evaluated.Materials and Methods:The release of drugs was studied by elution test.The anti-cancer activity of the drugs was assessed by MTT method.1x106 S180 cells was injected subcutaneously into 60 mice each.Seven days later,the mice were divided into 4 groups randomly.In three treatment groups,each anti-cancer agent bone cement 0.5ml was injected into tumor tissue of every mouse,respectively.The change of tumor size and the pathological examination were observed.Results:The release of those drugs could be detected within 10~24 days and their anti-cancer activity lasted about 12 or 24 hours on MTT method.The more volume of those drugs mixed with bone cement was,the more the release and anti-cancer activity was.In three treatment groups,the tumor size reduced gradually in 10 days.But still kepted on enlarging in control group.The tumor tissue around anti-cancer agent bone cement presented necrosis on the pathological examination.Conclusion:Those drugs released from bone cement have the anti-cancer effect.