B16细胞中FLT3表达的检测及FLT3-Ligand对B16细胞生长的抑制作用

目的：检测造血生长因子受体ＦＬＴ３ 在非造血来源的黑素瘤细胞系Ｂ１６ 中的表达，并研究其配基ＦＬＴ３－Ｌｉｇａｎｄ（ＦＬ）对Ｂ１６ 细胞的作用。方法：通过ＲＴ－ＰＣＲ检测Ｂ１６ 细胞中ＦＬＴ３ ｍ ＲＮＡ的表达，流式细胞仪检测ＦＬＴ３ 蛋白在Ｂ１６ 细胞表面的表达，在培养液中加入不同浓度的ＦＬ对Ｂ１６ 细胞进行培养。结果：ＦＬＴ３ 在Ｂ１６ 细胞中有ｍ ＲＮＡ 的表达，在Ｂ１６ 细胞表面有蛋白质的表达。ＦＬ对Ｂ１６ 细胞的生长有抑制作用，并且这种抑制作用是浓度依赖性的，在一定的浓度范围内随着ＦＬ浓度的增高而增强。结论：ＦＬＴ３ 的分布不仅仅局限于小鼠的造血系统和神经系统，也表达于上皮性的肿瘤细胞Ｂ１６ 中，并在其配基ＦＬ的作用下抑制Ｂ１６ 细胞的生长。

Expression of FLT3 tyrosine kinase receptor in B16 cell and suppressive effect of FLT3 Ligand on B16 cell

Objective: To analyze expression of hematopoietic growth factor receptor in mouse non hematopoiesis derived melanoma B16 cell line and effect of its ligand FLT3 Ligand on B16 cell. Methods: Expression of FLT3 mRNA was detected by RT PCR and expression of FLT3 at the surface of B16 was analyzed by FACS. Various doses of FLT3 Ligand were added to the culture medium to study its effect on B16 cell. Results: mRNA of FLT3 was expressed in B16 cell and FLT3 was expressed at the surface of B16 cell.The growth of B16 was suppressed by FLT3 Ligand and the effect was dose dependent. Conclusion: The results suggest that FLT3 be not only expressed in hematopoietic tissues,but also distributed in malignant epithelial cell line B16 and play a role in its growth.