首页 | 本学科首页   官方微博 | 高级检索  
     

神经生长因子在小鼠病毒性心肌炎心肌中表达的意义
引用本文:于小华,张新刚,王时俊,陈瑞珍,杨英珍,李双杰. 神经生长因子在小鼠病毒性心肌炎心肌中表达的意义[J]. 临床儿科杂志, 2006, 24(5): 402-405
作者姓名:于小华  张新刚  王时俊  陈瑞珍  杨英珍  李双杰
作者单位:南华大学附属第一医院儿科,湖南,衡阳,421001;复旦大学附属中山医院,上海市心血管病研究所,卫生部病毒性心脏病重点实验室
基金项目:国家自然科学基金资助(项目编号:30271665)
摘    要:目的探讨病毒性心肌炎(VMC)发病过程中,心肌组织神经生长因子(NGF)表达的动态变化及发病机制中作用。方法70只4周龄雄性BALB/C小鼠腹腔接种0.1ml的CVB3建立VMC模型,10只同周龄同品系小鼠腹腔接种Eagle’s液作为对照组。VMC组于接种后3、7、15、30d,对照组于30d处死,采用RT-PCR、免疫组化检测心肌NGF mRNA及蛋白表达,并对NGF表达水平与心肌病变积分进行相关性分析。结果VMC组3~30d各时点心肌NGF mRNA及蛋白表达水平均明显增高,以7d时为最高,与对照组比较,差异均有显著性(P<0.05或0.01),而且NGF蛋白表达水平与心肌病变积分呈显著正相关(r=0.90,P<0.05)。结论NGF过度表达可能在病毒性心肌炎的发病机制中起重要作用。

关 键 词:神经生长因子  心肌炎  病毒  小鼠
文章编号:1000-3606(2006)05-402-04
收稿时间:2005-03-10
修稿时间:2005-03-10

Significance of nerve growth factor expression in myocardial tissue of mice with viral myocarditis
YU Xiao-hua,ZHANG Xin-gang,WANG Shi-jun,CHEN Ru-zhen,YANG Ying-zhen,LI Shuan-jie. Significance of nerve growth factor expression in myocardial tissue of mice with viral myocarditis[J]. The Journal of Clinical Pediatrics, 2006, 24(5): 402-405
Authors:YU Xiao-hua  ZHANG Xin-gang  WANG Shi-jun  CHEN Ru-zhen  YANG Ying-zhen  LI Shuan-jie
Affiliation:Department of Pediatrics, the First Affliated Hospital, Nanhua University, Hunan Hengyang 21001, China
Abstract:Objective To investigate the dynamic changes and significance of nerve growth factor (NGF)expression in myocardial tissues of mice with viral myocarditis(VMC). Methods Eighty four-week- old male BALB/C mice were divided into two groups randomly. Mice in the VMC group (n=70) were ino- culated intraperitoneally with 0.1ml of CVB3,while mice in the control group (n=10) were treated with 0.1ml of Eagle's solution. Ten infected mice were sacrificed on day 3,7,15 or 30 respectively after inoculation,and the control mice were killed on day 30. The expression levels of myocardial NGF mRNA and protein were examined with RT-PCR and immunohistochemistry. In addition,the correlationship between the expression levels of NGF and myocardial histopathological scores was analyzed. Results In comparison with those in control group,the expression levels of myocardial NGF mRNA and protein from day 3 to 30 in VMC group increased significantly and reached the peak level on day 7 after inoculation (P<0.05 or 0.01). Furthermore,the NGF protein expression levels showed significantly positive correlationship with the myocardial histopathologic scores(r=0.90,P<0.05). Conclusions The excessive expression of NGF may play an important role in the pathogenesis of viral myocarditis.
Keywords:nerve growth factor  myocarditis  virus  mice
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号