应用胶体金SEA—DIPSTICK法检测日本血吸虫病血清抗体的研究

目的：建立一种快速、简便、实用的检测日本血吸虫病血清抗体的Dipstick方法。方法：应用胶体金SEA－dipstick法检测日本血吸虫病血清抗体。结果：用该法检测急性血吸虫病血清28人份和慢性血吸虫病血清297人份，其阳性检出率分别为100％和97.13％。在518例正常人和573例其它4种寄生虫病人血清中有0.19％假阳性和2.4％交叉反应。与Dot-ELISA比较，经卡方检验表明两者的敏感性无显著性差异，而SEA－dipstick法的特异性较Dot-ELISA强。结论：胶体金SEA－dipstick法检测日本血吸虫病血清抗体有较高的敏感性和特异性，并且方法快速，操作简便，不需特殊仪器设备，稳定性高，重复性好，具有广泛的 应用前景。

STUDY ON THE DETECTION OFSERUM ANTIBODY IN SERA OF PATIENTS WITH SCHISTOSOMIASIS JAPONICA BY COLLOIDAL GOLD SEA-DIPSTICK

Aim To develop an accurate,rapid,simple and applicable assay for the diagmosis of Schistosomiasis japonica.Method A time-saving,reliable colloidal gold SEA-dipstik assay was applied to detect serum antibody of schistosomiasis japonica.Results All of 28 sera from patients with accute schistosomiasis showed positive reaction while 289 of 297(97.31%) sera from chronic schistosomiasis were positive by SEA-dipstick assay.1 of 518(0.19%) sera from normal population gave false positive reaction 14 of 573(2.4%) cases of various other parasitic diseases,including paragonimiasis,clonorchiasis,cysticercosis,and trichinellosis,showed cross reaction.SEA-dispstick assay showed similar sensivity and higher specificity as parallel testing by Dot-LISA (P<005).Conclusion Colloidal gold SEA-dipstick assay is not only rapid and simple,but also sensitive adn specific for the detection of serum antibody of schistosomiasis japonica.It will be a practical immunological assay for the diagnosis of schistosomiasis in the future.