乙醇处理与大鼠脑内多巴胺能体系的关系

目的观察乙醇处理大鼠脑内酪氨酸羟化酶(TH)和多巴胺转运体(DAT)的表达变化,判断乙醇对脑内多巴胺(DA)能体系的影响。方法选取Wistar大鼠60只,随机分为对照组和乙醇处理组,每组30只,乙醇处理组大鼠以20%乙醇代替饮水饲养6个月;利用免疫组织化学、流式细胞术及免疫印迹等方法,分析乙醇处理大鼠有关脑区TH和DAT的表达改变。结果1.免疫细胞化学法研究发现,乙醇处理组大鼠脑内黑质(SN)-腹侧被盖区(VTA)、尾壳核(CPu)和伏核(NACC)TH的灰度值明显低于对照组(P0.05);尾壳核和伏核DAT的灰度值明显低于对照组(P0.05)。2.流式细胞术检测发现,乙醇处理组大鼠中脑TH的表达量明显高于对照组(P0.05)。3.免疫印迹检测发现,乙醇处理组大鼠所观察脑区TH和DAT与β-actin条带相对吸光度比值明显高于对照组(P0.05)。结论乙醇处理增加大鼠脑内TH和DAT的表达。

Relation of ethanol treatment with dopaminergic system in rat brain

Objective To study the effect of ethonal on the dopaminergic system by analyzing the altered expression of tyrosine hydroxylase (TH) and dopamine transporter (DAT) in the brain of ethanol-treated rats. Methods Sixty Wistar rats were selected and divided into control group and ethanol-treated group, 30 per group, the ethanol-treated rats were treated with 20% ethanol for 6 months. Immunohistochemistry, flow cytometry and Western blotting were used to analyze the altered expression of TH and DAT in the DA energic system in different brain regions of the ethanol treated rats. Results 1. Immunohistochemistry showed the mean gray value of TH in substantia nigra(SN)-ventrotegmental area (VTA), caudae putamen (Cpu) and nucleus accumbens (NACC), DAT in Cpu and NACC of the ethanol were smaller than those in control (P<0.05). 2. Flow cytometry showed the expression of TH in middle brain of the ethanol-treated rats increased significantly compared with the control(P<0.05). 3. Western blotting showed the ratio of IA of TH/β-actin and DAT/β-actin in different brain regions of the ethanol-treated rats were larger than those in control(P<0.05).Conclusion Ethanol treatment increases the expression of TH and DAT in rat brain.