Effect of a new synthetic serum replacement on insulin and somatostatin secretion from isolated rat pancreatic islets in long-term culture |
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Authors: | Vonen Barthold Bertheussen Kjell Giæver Anton K. Florholmen Jon Burhol Per G. |
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Affiliation: | (1) Laboratory of Gastroenterology, Department of Medicine, University Hospital of Tromsø, Tromsø, Norway;(2) Department of Surgery, University Hospital of Tromsø, Tromsø, Norway;(3) Institute of Medical Biology, University of Tromsø, Tromsø, Norway;(4) Department of Surgery, 9012 Regionsykehuset i Tromsø, Norway |
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Abstract: | Summary Serum contains insulin degrading components. We have evaluated the insulin and somatostatin secretion from isolated rat pancreatic islets during a 2-wk culture period using three different serum-containing media, and one serum-free medium with a synthetic serum replacement. Islets incubated in serum-free medium elicited significantly higher daily insulin and somatostatin secretions than islets incubated in the serum-containing media. After a 2-wk culture period, islets from the serum-free medium secreted significantly more insulin and somatostatin than islets cultured in other media when stimulated with 25 mmol/liter glucose together with 15 mmol/liter theophylline. We conclude that the serum-free medium is superior for long-term culture of rat pancreatic islets. |
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Keywords: | pancreatic islets culture serum-free insulin somatostain |
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