| 附睾精子冷冻保存对单精子胞浆内注射临床结局的影响 |
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| 引用本文： | 严正杰,杨晓玉,蔡令波,冯 婷,马 龙,陈 娟,王 伟,冒韵东,刘嘉茵. 附睾精子冷冻保存对单精子胞浆内注射临床结局的影响[J]. 南京医科大学学报(自然科学版), 2009, 29(7): 1000-1003 |
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| 作者姓名： | 严正杰 杨晓玉 蔡令波 冯 婷 马 龙 陈 娟 王 伟 冒韵东 刘嘉茵 |
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| 作者单位： | 南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029;南京医科大学第一附属医院临床生殖医学中心,江苏,南京,210029 |
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| 摘 要： | 目的:回顾性分析54例采用冷冻附睾精子行卵细胞浆内单精子注射(ICSI)的胚胎发育以及妊娠结局.方法:54例冷冻保存的附睾精子解冻复苏,采用ICSI法受精,观察胚胎发育形态,经72 h体外培养后移植,将282例采用新鲜附睾精子的ICSI结局为对照组.结果:正常受精率、卵裂率、优质胚胎率、妊娠率冻精组与新鲜组之间无显著差异(72.4%vs 74%,98.1%vs96.3%,51.7%vs 46.8%,42.6%vs 39.0%,P>0.05),两组均未出现新生儿畸形.冷冻附睾精子复苏率为65.9%.结论:冷冻附睾精子对胚胎发育以及妊娠结局无显著影响.
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| 关 键 词： | 经皮附睾穿刺取精术 冷冻 胚胎发育 单精子卵包浆内注射 |
| 收稿时间： | 2009-02-18 |
The outcome of ICSI with frozen-thawed sperm obtained by PESA on azoospermia patients |
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| YAN Zheng-jie,YANG Xiao-yu,CAI Ling-bo,FENG Ting,MA Long,CHEN Juan,WANG Wei,MAO Yun-dong and LIU Jia-yin. The outcome of ICSI with frozen-thawed sperm obtained by PESA on azoospermia patients[J]. Acta Universitatis Medicinalis Nanjing, 2009, 29(7): 1000-1003 |
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| Authors: | YAN Zheng-jie YANG Xiao-yu CAI Ling-bo FENG Ting MA Long CHEN Juan WANG Wei MAO Yun-dong LIU Jia-yin |
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| Affiliation: | Human Clinical Reproductive Medicine Center;the First Affiliated Hospital of NJMU;Nanjing 210029;China |
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| Abstract: | Objective:To investigate the effects of ICSI(intracytoplasmic sperm injection)cycles with frozen sperm obtained by PESA(percutaneous epididymal sperm aspiration)by retrospective study performed in 54 patients. Methods:Sperm from the 54 infertile males by PESA were frozen with conventional freezing method. After frozen-thawed and ICSI process,the fertilized oocytes were cultured in vitro for 72 hours and high quality embryos were transfered to the wives. 282 cases of fresh epidielymal sperms were tested as c... |
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| Keywords: | percutaneous epididymal sperm aspiration freezing embryo development intracytoplasmic sperm insemination |
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