| HPLC法测定加味苓桂术甘汤中桂皮醛的含量 |
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| 引用本文： | 蔡素华,温预关,莫玉泉.HPLC法测定加味苓桂术甘汤中桂皮醛的含量[J].海峡药学,2012,24(4):54-56. |
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| 作者姓名： | 蔡素华 温预关 莫玉泉 |
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| 作者单位： | 广东省广州市脑科医院药学部,广州,510370 |
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| 基金项目： | 广东省中医药局科技立项课题 |
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| 摘 要： | 目的建立测定加味苓桂术甘汤中桂皮醛含量的HPLC方法。方法采用Diamonsil C18柱(5μm,4.6mm×150mm),流动相为甲醇-0.03mol·L-1醋酸铵(50∶50),在290nm波长处检测,流速0.8mL·min-1,柱温40℃。结果桂皮醛在0.523～2.61μg·mL-1范围内呈良好的线性关系,r=0.9998;平均加样回收率为98.6%,RSD=0.95%(n=6)。测得加味苓桂术甘汤中桂皮醛的含量为8.10μg·mL-1。结论该方法灵敏、准确、重现性好,适用于测定加味苓桂术甘汤中桂皮醛的含量。
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| 关 键 词： | 加味苓桂术甘汤 桂皮醛 高效液相色谱法 |
Determination of cinnamaldehyde in Jiawei Lingguishugan decoction by HPLC |
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| CAI Su-hua
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WEN Yu-guan
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MO Yu-quan.Determination of cinnamaldehyde in Jiawei Lingguishugan decoction by HPLC[J].Strait Pharmaceutical Journal,2012,24(4):54-56. |
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| Authors: | CAI Su-hua WEN Yu-guan MO Yu-quan |
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| Institution: | (Department of Clinical Pharmacology,Guangzhou Brain Hospital,Guangzhou 510370,China) |
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| Abstract: | OBJECTIVE To develop an HPLC method for the determination of cinnamaldehyde in Jiawei Lingguishugan decoction.METHODS A Diamonsil C18 column（5μm,4.6mm×150mm）was used.The mobile phase was a mixture of methanol-0.03mol·L-1ammonium acetate（50∶50）.The detection wavelength was at 290nm.The flow rate was 0.8mL·min-1.The column temperature was 40℃.RESULTS The linear rang of cinnamaldehyde was 0.523～2.61μg·mL-1,r=0.9998.The average recovery was 98.6%,RSD=0.95%（n=6）.CONCLUSION The method is sensitive,accurate and the repeatable.It can be used for the determination of cinnamaldehyde in Jiawei Lingguizhugan decoction. |
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| Keywords: | Jiawei Lingguishugan decoction Cinnamaldehyde HPLC |
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