| 高效液相色谱法测定海南香茅中绿原酸的含量 |
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| 引用本文： | 王勇,魏娜,董静静. 高效液相色谱法测定海南香茅中绿原酸的含量[J]. 海南医学院学报, 2012, 18(4): 445-447,451 |
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| 作者姓名： | 王勇 魏娜 董静静 |
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| 作者单位： | 海南医学院药学院,海南海口,571101 |
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| 基金项目： | 海南省教育厅高等学校科学研究资助项目 |
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| 摘 要： | 目的:建立海南香茅中绿原酸的高效液相色谱含量测定方法。方法:采用高效液相色谱法,phe-nomenox luna C1 8色谱柱(250mm×4.6mm,5μm),以乙腈-水-冰醋酸(12∶88∶1)为流动相,流速为1.0mL/min,检测波长为327nm。结果:绿原酸在0.166 4～1.664 0μg范围内,峰面积与绿原酸的进样质量呈良好线性关系,线性方程为Y=1 334 267.8 X-2 392.2(r=0.999 9),平均回收率为101.58%,RSD为1.84%。结论:建立的高效液相色谱测定法简便、快速,重复性好,准确度高,可作为香茅药材的质量控制方法。
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| 关 键 词： | 香茅 绿原酸 含量 高效液相色谱法 |
Determination of chlorogenic acid in the herb of Cymbopogon citrus from Hainan by HPLC |
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| WANG Yong , WEI Na , DONG Jing-jing. Determination of chlorogenic acid in the herb of Cymbopogon citrus from Hainan by HPLC[J]. Journal of Hainan Medical College, 2012, 18(4): 445-447,451 |
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| Authors: | WANG Yong WEI Na DONG Jing-jing |
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| Affiliation: | (School of Pharmaceutical Sciences,Hainan Medical University,Haikou 571101,China) |
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| Abstract: | Objective: To establish a method for the determination of chlorogenic acid in the herb of Cymbopogon citrus from Hainan by HPLC.Methods:HPLC determination was carried out on a phenomenox luna C18 column(250 mm × 4.6 mm,5 μm) with acetonitrile-water-acetic acid(12∶88∶1) as mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was set as 240 nm.Results:There was good linear relationship for chlorogenic acid in the range of 0.166 4-1.664 0 μg.The linearity equation was Y=1 334 267.8 X-2 392.2(r=0.999 9).The average recovery was 101.58% and RSD was 1.84%.Conclusions:The established method is simple,quick,reproducible and accurate,which can be used for the quality control of Cymbopogon citrus. |
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| Keywords: | Cymbopogon citrus Chlorogenic acid Assay HPLC |
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