胆囊收缩素促大鼠坐骨神经再生的电生理检测及意义

目的:观察八肽胆囊收缩素(CCK-8)促大鼠坐骨神经损伤修复的效果。方法:24只健康SD大鼠随机分成实验组(CCK-8治疗组)和对照组(生理盐水治疗组)两组,每组12只,采用右侧坐骨神经离断后缝合外膜的损伤模型。CCK-8组大鼠腹腔注射CCK-8(8nmol/kg),每天一次,连续7d,对照组则用生理盐水代替CCK-8,在术后第12周检测大鼠坐骨神经干动作电位传导速度及肌电图。结果:实验组行为方面比对照组恢复早。术后第12周,两组大鼠坐骨神经干动作电位传导速度、肌电图潜伏期及波幅差异均有统计学意义(t分别为11.60、-8.83、4.51,P均<0.001),实验组的神经干动作电位传导速度快,肌电图潜伏期短、波幅值大,实验组恢复情况优于对照组。结论:CCK-8能有效地促进周围神经再生。

Electrophysiological determination of regeneration of sciatic nerve by Cholecystokinin-octapeptide (CCK-8) in rats and its significance

Objective: To assess the regeneration of the sciatic nerve by Cholecystokinin-octapeptide(CCK-8) in rats by electrophysiological determination.Methods: A total of 24 SD rats were randomly divided into test group(CCK-8-treated group) and control group(physiological saline-treated group) with 12 rats in each.The right sciatic nerve was transected and epineurium sutured to establish rat models.CCK-8 treated group were injected with CCK-8 at the dose of 8 nmol/kg intraperitoneally,once per day,for 7 days.The control group received isotonic saline at the same dosage.After 12 weeks,the action potential and electromyogram for sciatic nerve in vivo were recorded to analyze nervous conduct velocity.Results:Regeneration of the sciatic nerve of the test group was quicker than that of the control group.After 12 weeks of treatment,the test group showed significant quicker conduct velocity,larger wave shape,but shorter potential phase than the control group(t=11.60、-8.83、4.51,P all <0.001).The recovery of test group was superior to that of the control group.Conclusion: CCK-8 can promote nervous regeneration.