| 靶向mdr1b/mdr1a基因siRNA逆转T24/ADM多药耐药性研究 |
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| 引用本文： | 刘正清,姚启盛,杨勇,陈从波,龚小新,黄力,王黎.靶向mdr1b/mdr1a基因siRNA逆转T24/ADM多药耐药性研究[J].海南医学院学报,2012,18(7):865-868. |
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| 作者姓名： | 刘正清 姚启盛 杨勇 陈从波 龚小新 黄力 王黎 |
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| 作者单位： | 湖北医药学院附属太和医院泌尿外科,湖北十堰,442000 |
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| 基金项目： | 湖北省教育厅科学技术研究计划重点项目 |
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| 摘 要： | 目的:研究mdr1b/a基因siRNA(small interfering RNA)逆转膀胱癌的多药耐药性。方法:设计mdr1b/a基因的siRNA,转染膀胱癌T24/ADM细胞,用RT-PCR分析mRNA的表达,Rh123分析P-gp活性,流式细胞仪检测细胞内阿霉素积累量。结果:siRNA能明显地逆转T24/ADM细胞的多药耐药,使mdr1b/a基因的mRNA表达水平下调,细胞内阿霉素积累量显著增加(P<0.05)。结论:mdr1b/a基因siRNA能够逆转膀胱癌的多药耐药性。
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| 关 键 词： | siRNA mdr1b/a基因 多药耐药 膀胱癌 |
Effect of mdr1b/mdr1a siRNA on multidrug resistance of bladder carcinoma cell line T24/ADM |
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| LIU Zheng-qing
,
YAO Qi-sheng
,
YANG Yong
,
CHEN Cong-bo
,
GONG Xiao-xin
,
HUANG Li
,
WANG Li.Effect of mdr1b/mdr1a siRNA on multidrug resistance of bladder carcinoma cell line T24/ADM[J].Journal of Hainan Medical College,2012,18(7):865-868. |
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| Authors: | LIU Zheng-qing YAO Qi-sheng YANG Yong CHEN Cong-bo GONG Xiao-xin HUANG Li WANG Li |
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| Institution: | (Department of Urological Surgery,Taihe Hospital Affiliated to Hubei College of Medicine,Shiyan 442000,China) |
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| Abstract: | Objective: To investigate the effect of mdr1b/mdr1a siRNA on multidrug resistance of bladder carcinoma cell line T24/ADM.Methods:The proper mdr1b/a siRNA was designed,synthesized and transfected into bladder carcinoma cell line T24/ADM.Expression level of mdr1b/a mRNA was determined by PCR.The activity of Pgp was measured by Rhodamine 123 efflux assay.The intracellular accumulation of ADM was assayed with flow cytometry.Results:After treated with siRNA,the expression level of mdr1b/a mRNA in T24/ADM decreased obviously,and the intracellular accumulation of ADM was increased significantly(P<0.05).Conclusions:The mdr1b/asiRNA can reverse the multidrug resistance of bladder carcinoma cell line T24/ADM. |
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| Keywords: | siRNA mdr1b/a gene Multidrug resistance Bladder carcinoma cell |
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