| HPLC法测定复方甘草颗粒中甘草酸的含量 |
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| 引用本文： | 王梅平.HPLC法测定复方甘草颗粒中甘草酸的含量[J].海峡药学,2012,24(5):42-44. |
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| 作者姓名： | 王梅平 |
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| 作者单位： | 浙江省富阳市妇幼保健院中药房,富阳,311400 |
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| 摘 要： | 目的 建立对复方甘草颗粒中甘草酸的含量测定方法.方法 高效液相色谱法,色谱条件:ZORBAX XDB-C18(150mm×4.6mm,5μm),柱温25℃;流动相为甲醇- 0.2mol·L-1醋酸胺溶液-冰醋酸(67∶33∶1);流速0.8mL·min-1;检测波长:250nm.结果 甘草酸在0.19～1.88μg范围内,线性关系良好,回归方程为Y=9.10×105X-8.75×103(r=0.9999),平均加样回收率为98.50%,RS D=1.35%(n=5).结论 本法稳定可靠、简便、快速、重现性好.
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| 关 键 词： | 高效液相色谱法 甘草酸 含量测定 复方甘草颗粒 |
Determination of glycyrrhizic acid in Granulae Glycyrrhizae composi tae by HPLC |
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| WANG Mei-Ping.Determination of glycyrrhizic acid in Granulae Glycyrrhizae composi tae by HPLC[J].Strait Pharmaceutical Journal,2012,24(5):42-44. |
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| Authors: | WANG Mei-Ping |
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| Institution: | WANG Mei-Ping (Department of TCM pharmacy Fuyang Health Hospital For Women And Children Fuyang 311400) |
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| Abstract: | OBJECTIVE An HPLC method was developed for the determination of Glycyrrhizic Acid in Granulae Glycyrrhizae Compositae.METHODS The analysis was carried on a column of ZORBAX XDB-Cta（150mm×4.6mm,5μm） at 25℃ ; The mobile phase was consisted of methanol-0.2mol·L- 1 ammonium acetate solutionacetic acid glacial （67 ： 33 ： 1 ） .The flow rate was 0.8mL · min-1 .The detection wavelength was at 250nm. RESULTS A good linearity can be found with in the range of 0.19-- 1.88μg. The average recovery rate was 98.50 % （r = 0.9999）, RSD = 1.35 96 （n = 5） for determination of Glycyrrhizic Acid in Granulae Glycyrrhizae Compositae. CONCLUSION The method is simple, rapid, and reproducible for determination of Glycyrrhizic Acid in Granulae Glycyrrhizae Compositae. |
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| Keywords: | Glycyrrhizic Acid HPLC Granulae Glycyrrhizae Compositae |
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