乙型肝炎血清标志物四种检测方法的对比分析

目的:应用酶联免疫吸附试验(ELISA)、胶体金免疫层析法(GICA)、时间分辨免疫荧光法(TR-FIA)、化学发光法(CLIA)检测乙型肝炎血清标志物[乙型肝炎表面抗原(HbsAg)、乙型肝炎表面抗体(抗Hbs)、乙型肝炎e抗原(HbeAg)、乙型肝炎e抗体(抗Hbe)、乙型肝炎核心抗体(抗Hbc)],评价不同方法学检测结果之间是否具有一致性。方法:用GICA、ELISA、TRFIA、CLIA分别检测50例乙型肝炎病毒(HBV)感染者和100名正常体检者的乙型肝炎血清标志物。以CLIA作为参考方法,通过Kap-pa检验评价不同方法学检测结果之间的一致程度。结果:4种方法检测乙型肝炎血清标志物的结果具有高度以上一致性,CLIA在检测敏感性上更有优势。结论:目前广泛使用的检测乙型肝炎血清标志物的方法具有较好的一致性,为临床实验室方法学选择提供了依据。

Comparison of four detection methods for hepatitis B serum markers

Objective: To evaluate the concordance of enzyme-linked immunosorbent assay(ELISA),Gold-immunochromatography(GICA),time-resolved fluorescence immunoassay(TRFIA) and chemiluminescence immunoassay(CLIA) for detecting serum hepatitis B vivus(HBV) markers including hepatitis B surface antigen(HbsAg),antibody to HbsAg(anti-Hbs),hepatitis B e antigen(HbeAg),antibody to HbeAg(anti-Hbe) and antibody to hepatitis B core antigen(anti-Hbc).Methods:HBV markers of 50 patients infected with HBV and 50 healthy subjects were detected by ELISA,GICA,TRFIA and CLIA.Detecting results of CLIA was performed as reference method,and Kappa test was used for determining the concordance of the 4 different methods.Results:It revealed high concordance between the 4 methods and CLIA method showed better sensitivity than the other methods.Conclusions:The currently used detecting methods showed good concordance for screening HBV markers.It provides evidence for detecting method choice.