| HPLC法测定苦参素注射液中枸橼酸含量 |
| |
| 引用本文： | 罗嵚,谢敏,吴春敏.HPLC法测定苦参素注射液中枸橼酸含量[J].海峡药学,2012,24(5):38-40. |
| |
| 作者姓名： | 罗嵚 谢敏 吴春敏 |
| |
| 作者单位： | 1. 福建中医药大学,福州,350108;福建省药品检验所,福州,350001
2. 福建省药品检验所,福州,350001 |
| |
| 摘 要： | 目的 建立苦参素注射液中枸橼酸的高效液相色谱检测方法.方法 采用Ecli pse XDB-C18色谱柱(4.60×150mm,5μm),以乙腈-0.02mol·L-1磷酸二氢钾(含0.2%辛烷磺酸钠,用磷酸调节pH至2.5)为流动相进行梯度洗脱,检测波长为210nm,流速为0.5mL·min-1.结果 枸橼酸在0.04356mg·mL- 1～1.089mg·mL-1范围内线性关系良好(r=1),平均回收率为100.0%.结论 该方法简便、准确、灵敏、重复性好,可作为该制剂质量控制方法.
|
| 关 键 词： | HPLC 枸橼酸 含量 苦参素注射液 |
Determination of Citric Acid in Kushensu Injection by HPLC |
| |
| LUO Qin
,
XIE Min
,
WU Chun-min.Determination of Citric Acid in Kushensu Injection by HPLC[J].Strait Pharmaceutical Journal,2012,24(5):38-40. |
| |
| Authors: | LUO Qin XIE Min WU Chun-min |
| |
| Institution: | 1.Fujian University of Traditional Chinese Medicine, Fuzhou 350108,China;2. Fujian Institute for Drug Control, Fuzhou 350001, China) |
| |
| Abstract: | OBJECTIVE To develop an HPLC method for determination of Citric Acid in Matrin Injection.METHODS The HPLC was performed on a Eclipse XDB-Ct8 column（4.60 × 150mm, 5μm）, the mobile phase was acetonitrile-0.02mol· L- 1 potassium dihydrogen phosphate （0.2 % 1-octane sulfonic acid sodium salt, adjusted to pH 2.5 with phosphoric acid）, the detection wavelength was 210nm, the flow rate 0.5mL·min-1 ~ RESULTS The Citric Acid linear range was 0. 04356mg·mL- 1 1.089mg· mL- t, and the average recovery was 100.0 %. CONCLUSION The method is convenient, accurate, sensitive and repeatability, which can be used to as a quality control method for Matrin Injection. |
| |
| Keywords: | H PLC Matrin Injection Citric Acid Determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
