基因芯片筛选妊娠高血压综合征胎盘差异表达基因

目的了解妊高征患者胎盘与正常胎盘在分子水平的差异,为研究妊高征病因提供新的线索.方法以混合的4例正常胎盘总RNA为对照,用cy5dUTP和cy3dUTP分别标记对照cDNA和妊高征胎盘cDNA.用4000点cDNA表达谱芯片检查4例妊高征患者胎盘基因表达的改变.结果在4次芯片杂交过程中,分别有58-131条不等的基因发生差异表达,其中出现2次以上重复一致的异常表达基因共22条:表达增高的有13条,表达降低的有9条.结论应用cDNA表达谱芯片可快捷、高通量地筛选出妊高征胎盘可能的致病基因,为研究妊高征的病因提供新的思路和线索.

Microarray analysis of genes differentially expressed in placentas of pregnancy - induced hypertension patients

Objective: To uncover new clue for the research of the etiology of pregnancy-induced hypertension (PIH) by testing the gene expression difference between preeclamptic placentas and normal ones. Methods: mRNA level of 4 PIH placentas were examined using 4,000 feature cDNA Microarray in comparison with the pooled control consisting of total RNA from 4 cases of PIH placentas after the control cDNA and experimental cDNA were labeled by cy3 and cy5 respectively. Results: 58 to 131 genes were found down or up -regulated in 4 runs of hybridization. Among the differentially expressed genes, 22 genes, including genes encoding secreted protein ADRP, CYR61, EPI and HIF2, have the concordance in at least 2 cases were up-regulated or down-regulated. Conclution: cDNA Microarray is a high throughtput and time-saving method to monitor the altered gene expression and the result could provide interesting clue and strategy for the etiological research of PIH.