| 超氧化物歧化酶活力测定改进法 |
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| 引用本文： | 超氧化物歧化酶活力测定改进法[J]. 中国药科大学学报, 1988, (3): 213-215. |
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| 作者姓名： | 胡梅清 李继珩 吴梧桐 |
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| 作者单位： | 南京铁道医学院(胡梅清)，中国药科大学生物化学教研室(李继珩)，中国药科大学生物化学教研室(吴梧桐) |
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| 摘 要： | 超氧化物歧化酶(EC1.15.1.1,简称SOD)是一种具有很强抗炎作用的消炎酶,其活力测定方法已有多种,但操作均较复杂且重现性较差。本文报道一种简易可行的改良法,应用牛血SOD标准品建立不同酶活力对邻苯三酚自氧化作用抑制率的标准曲线,在相
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| 关 键 词： | 人超氧化物歧化酶 自氧化速率 同工作用 |
IMPROVED METHOD FOR DETERMINATION OF SUPEROXIDE DISMUTASE ACTIVITIES |
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| IMPROVED METHOD FOR DETERMINATION OF SUPEROXIDE DISMUTASE ACTIVITIES[J]. Journal of China Pharmaceutical University, 1988, (3): 213-215. |
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| Authors: | Hu Meiqing Li Jiheng and Wu Wutong |
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| Affiliation: | Hu Meiqing~ Li Jiheng and Wu Wutong |
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| Abstract: | Superoxide dismutase(SOD) is an antiphlogistic enzyme which possesses a strong antiinflamination. The reproduction of analytical results is not very desirable and it is more difficult to compare the results from different methods, although a few methods have been used for determination of SOD. An improved method was described: the working curve was established by standard SOD from bovine, using the same procedure, and SOD activities from samples would be got from working curve. The results indicated that the method is rapid and simple to operate, its accuracy and the reproduction are better, and the results from different assay methods can be also compared by the method. The effects of temperature and pH on determination were disscused as well. At the same time, human SOD activities were determined and isodynamic properties of SOD (from human blood and boyine blood) were observed by the method. |
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| Keywords: | Human superoxide dismutase (hSOD) Rate of autooxydation Isodynamication |
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