| 抗原负载的树突状细胞介导的抗慢性粒细胞白血病作用 |
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| 引用本文： | 主鸿鹄,潘秀英,刘军权,陈复兴,黄一虹,何徐彭,徐开林.抗原负载的树突状细胞介导的抗慢性粒细胞白血病作用[J].白血病．淋巴瘤,2001,10(5):269-271. |
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| 作者姓名： | 主鸿鹄 潘秀英 刘军权 陈复兴 黄一虹 何徐彭 徐开林 |
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| 作者单位： | 1. 徐州医学院附属医院,江苏,徐州,221002
2. 解放军第97医院,江苏,徐州,221006 |
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| 摘 要： | 目的：研究慢性粒细胞白血病（CML）细胞冻融抗原(CLA)负载的树突状细胞（DC）对特异性抗白血病T细胞的作用。方法：将健康人外周血单个核细胞来源的DC在体外用CLA负载，再与CML患者的经细胞因子诱导的杀伤细胞（CIK）共同培养，应用乳酸氢酶释放法观察其对自身CML细胞杀伤活性，与未负载的DC与CIK共培养组、CIK组分别进行比较。结果：DC经抗原负载后介导的特异性抗白血病T细胞对CML细胞杀伤活性明显增强。结论：用CLA负载可进一步提高DC介导的特异性抗白血病T细胞对CML细胞的杀伤活性。
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| 关 键 词： | 树突状细胞 慢性粒细胞白血病 细胞因子诱导的杀伤细胞 免疫治疗 |
| 文章编号： | 1009-9921(2001)05-0269-03 |
| 修稿时间： | 2001年2月20日 |
THE IMMUNOSTIMULATORY EFFECT OF DENDRITIC CELLS BY PULSED WITH CHRONIC MYELOGENOUS LEUKEMIC LYSATE ANTIGEN |
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| ZHU Hong hu,PAN Xiu ying,LIU Jun quan,et al..THE IMMUNOSTIMULATORY EFFECT OF DENDRITIC CELLS BY PULSED WITH CHRONIC MYELOGENOUS LEUKEMIC LYSATE ANTIGEN[J].Journal of Leukemia & Lymphoma,2001,10(5):269-271. |
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| Authors: | ZHU Hong hu PAN Xiu ying LIU Jun quan |
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| Institution: | ZHU Hong hu,PAN Xiu ying,LIU Jun quan,et al. Affiliated Hospital of Xuzhou Medical College |
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| Abstract: | Objective:To investigate the immunostimulatory effect of dendritic cells (DCs) pulsed with chronic myelogenous leukemic (CML) lysate antigen (CLA).Methods:DCs from normal donors were pulsed by CLA,and cocultured with cytokines induced killer cells (CIKs) from CML patients.The cytotoxic activity was measured using lactate dehydrogenase release assay.Results:Coculture of CIKs with DCs led to a significant increase in cytotoxic activity compared with CIK.The cytotoxic activity could be further increased using DCs pulsed with CLA.Conclusion:DCs pulsed with CLA can increase the cytotoxic activity of immunologic effector cells against CML cells.DCs pulsed with CLA may have an important effect on immunotherapy protocols for patients with CML. |
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| Keywords: | Dendritic cells Chronic myelogenous leukemia Cytokines induced killer cells Immunotherapy |
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