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| 圣和散逆转胃癌SGC7901/VCR细胞多药耐药的研究 | |
| 引用本文： | 夏跃胜,王建华,吴永忠,王欢. 圣和散逆转胃癌SGC7901/VCR细胞多药耐药的研究[J]. 中医杂志, 2006, 47(6): 459-460 |
| 作者姓名： | 夏跃胜 王建华 吴永忠 王欢 |
| 作者单位： | 1. 永济市肝胆胃病医院,山西省永济市中山东街39号,044500 2. 中国人民解放军第四军医大学唐都医院 3. 山西中医学院 |
| 摘 要： | 目的探讨中药圣和散逆转胃癌耐药细胞SGC7901/VCR多药耐药的机制。方法圣和散(浓度2.5、5和10mg/ml)处理胃癌耐药细胞SGC7901/VCR,用流式细胞技术检测P-糖蛋白(P-gp)表达,免疫组织化学SABC法及图像分析技术检测B细胞淋巴瘤/白血病基因-2(Bcl-2)。结果实验组SGC7901/VCR细胞Bcl-2表达A值(121.34±0.16)明显高于对照组(104.37±0.089)(P<0.05),Bcl-2表达减少,P-gp表达也明显降低。结论圣和散能逆转胃癌耐药细胞SGC7901/VCR多药耐药,其作用可能与调节Bcl-2和P-gp表达有关。 |
| 关 键 词： | 胃肿瘤/中医药疗法 抗药性,多药/中医药疗法 @圣和散 |
| 收稿时间： | 2005-06-14 |
| 修稿时间： | 2005-06-142005-11-10 |
Study on Shenghe Powder for Reversion of Multidrug Resistance of SGC7901/VCR Cells of Gastric Cancer |
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| Xia Yuesheng,Wang Jianhua,Wu Yongzhong,et al.. Study on Shenghe Powder for Reversion of Multidrug Resistance of SGC7901/VCR Cells of Gastric Cancer[J]. Journal of Traditional Chinese Medicine, 2006, 47(6): 459-460 | |
| Authors: | Xia Yuesheng Wang Jianhua Wu Yongzhong et al. |
| Affiliation: | Xia Yuesheng1,Wang Jianhua1,Wu Yongzhong2,et al. |
| Abstract: | Objective:To explore the mechanism of Shenghe Powder(SHP) in reversion of multidrug resistence of SGC7901/VCR cells of gastric cancer.Methods:Gastric cancer drug-resistance cells SGC7901/VCR were treated with SHP at concentrations of 2.5,5.0 and 10mg/ml.The expression of P-glycoprotein(P-gp) was detected with flowcytometry and B-cell lymphoma/leukemia-2(Bcl-2) protein was examined by immunohistochemical SABC method and imaging analysis.Results:In SHP treated group,A value of Bcl-2 in SGC7901/VCR cells was significantly higher than that in the control group(P<0.05),and the expression of Bcl-2 and P-gp was significantly down-regulated.Conclusion:SHP can reverse the multidrug resistance of SGC7901/VCR,which is possibly related with the changes of the expressions of Bcl-2 and P-gp. |
| Keywords: | Gastric tumor/TCM therapy Resistance,multidrug/TCM therapy @ Shenghe Powder |
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