| 拓扑替康诱导肺癌细胞HTB-56凋亡早期线粒体膜电位的变化 |
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| 引用本文： | 高丰,刘思思,韩佩妍,王武龙,邹瑞,王刚,曹阳,吴海歌,王若雨,高文斌. 拓扑替康诱导肺癌细胞HTB-56凋亡早期线粒体膜电位的变化[J]. 中华肺部疾病杂志(电子版), 2013, 6(1): 51-53 |
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| 作者姓名： | 高丰 刘思思 韩佩妍 王武龙 邹瑞 王刚 曹阳 吴海歌 王若雨 高文斌 |
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| 作者单位： | 大连大学研究生院;包头医学院第二附属医院放疗科;大连大学附属中山医院肿瘤科;大连大学生命科学与技术学院 |
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| 基金项目： | 2009年大连市科技局科技计划项目(项目编号:2009E11SF231);2009年大连大学附属中山医院院长基金项目 |
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| 摘 要： | 目的探讨拓扑替康诱导肺癌细胞HTB-56凋亡早期线粒体膜电位的改变。方法选择人肺癌细胞HTB-56细胞株，JC-1荧光染色标记线粒体，用早期流式细胞仪检测拓扑替康作用于HTB．56细胞不同时间细胞线粒体膜电位。采用琼脂糖凝胶电泳检测拓扑替康作用不同时间引起HTB-56细胞凋亡情况。结果经拓扑替康处理后48h、72hHTB-56细胞出现了明显的凋亡带，对照组未见明显亮带影。4h后细胞线粒体膜电位下降，8h后细胞膜电位明显下降，且下降程度随时间延长而增大。且药物组绿／红荧光比值与对照组比较均有显著性差异（P〈0．05）。结论拓扑替康诱导肺癌细胞HTB-56可使之凋亡，其机制可能与线粒体膜电位在凋亡极早期发生改变有关。
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| 关 键 词： | 支气管肺癌 拓扑替康 细胞凋亡 线粒体,膜电位 |
Mitochondrial membrane potential change in topotecan inducing apoptosis of lung cancer cells HTB-56 |
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| GAO Feng,LIU Si-si,HAN Pei-yan,WANG Wu-long,ZHOU Rui,WANG Gang,CAO Yang,WU Hai-ge,WANG Ruo-yü,GAO Wen-bin. Mitochondrial membrane potential change in topotecan inducing apoptosis of lung cancer cells HTB-56[J]. Chinese Journal of lung Disease（Electronic Edition）, 2013, 6(1): 51-53 |
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| Authors: | GAO Feng LIU Si-si HAN Pei-yan WANG Wu-long ZHOU Rui WANG Gang CAO Yang WU Hai-ge WANG Ruo-yü GAO Wen-bin |
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| Affiliation: | 1 Graduate School,Dalian University,Dalian 116622,China;2 Radiology Department,the Second Affiliated Hospital of Baotou Medical College,Dalian 116622 China;3 Omcology Department,Zhongshan Hospital Affiliated to Dalian University,Dalian 116622,China;4 School of Life Science and Technology,Dalian University Dalian,116622 China) |
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| Abstract: | Objective To investigate the mitochondrial membrane potential changes in topotecan inducing apoptosis in lung cancer cells HTB-56. Methods Mitochondrial of lung cancer cells HTB-56 was labeled by JC-1 fluorescence dye, mitocbondrial membrane potential was detected using flow cytometry after topotecan inducing apoptosis of lung cancer cells HTB-56 at different times, topotecan inducing apoptosis of lung cancer cells HTB-56 at different times was detected by agarose gel electrophoresis. Results The HTB-56 cells treated by topotecan revealed a obvious bright band in 48 hours and 72 hours, mitochondrial membrane potential decreased in 4 hours or more in 8 hours, and decreased over time. Green/red fluorescence ratio has a significant difference between drug group and control group （ P 〈 0. 05 ）. Conclusion Topotecan can induce apoptosis of lung cancer cells HTB-56 , the mechanism of apoptosis can be related to mitochondrial membrane potential changes in very early in apoptosis. |
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| Keywords: | Bronchogenic carcinoma Topoteean Cells apoptosis Mitochondrial, membrane potential |
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