Preliminary Study on the Cooperation of IL-6 and mB7-1 in the Induction of Effective Antitumor Immunity In Vitro

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Preliminary Study on the Cooperation of IL 6 and mB7 1 in the Induction of Effective Antitumor Immunity In Vitro *

To find a new way for gene therapy against tumors with weak immunogenicity, the effect of mB7 1 costimulation alone, or combined with IL 6, in inducing antitumor immunity in vitro was investigated. It was found that mB7 1 cDNA transfected B16 cells (B16 mB7 1) induced the expansion of effector lymphocytes and the generation of specific lytic activity more effectively than wild type B16 melanoma cells (B16 wt) or mock transfected B16 cells (B16 neo) did. ( P <0.01), IL 6 could effectively stimulate lymphocytes proliferation, but failed to enhance its cytotoxicity, while the combination of mB7 1 and IL 6 increased both lymphocyte proliferative response and T cell mediated cytotoxicity more significantly than B7 1 or IL 6 did alone ( P <0.01) . It was inferred that the costimulatory molecule B7 1 is required for the activation and proliferation of T lymphocytes; the expression of mB7 1 in tumor cells could increase their immunogenicity and induce effective antitumor immune response, and the combination of B7 1 and IL 6 could induce more effective antitumor immunity, indicating that cooperation of IL 6 and mB7 1 plays a role in T lymphocyte activation.