基于生物信息学分析细胞周期蛋白依赖蛋白激酶5表达与胃癌免疫浸润细胞的相关性

目的 探讨细胞周期蛋白依赖蛋白激酶5(CDK5)表达与胃癌免疫浸润细胞的关系.方法 采用基因富集分析(GSEA)及单样本基因富集分析(ssGSEA)对免疫相关基因集进行富集评分,采用CIBERSORT网站对免疫细胞组分进行评分.收集36例行胃癌根治术的患者的胃癌组织,采用免疫组织化学法检测CDK5及CD8表达水平.结果...

Correlation between cyclin-dependent protein kinase 5 and gastric cancer immune infiltrating cells based on bioinformatics analysis

Objective To explore the relationship between cyclin-dependent protein kinase 5(CDK5)and gastric cancer immune infiltrating cells.Method Gene set enrichment analysis(GSEA)and single-sample gene set enrichment analysis(ssGSEA)were used to enrich and score the immune-related gene sets.The CIBERSORT website was used to score the immune cell components.A total of 36 patients who underwent radical gastrectomy were selected and their cancer tissues were used to detect the expression levels of CDK5 and CD8 by immunohistochemical(IHC)staining assay.Result Conclusion The number of infiltration of CD8⁺T lymphocytes and memory B lymphocytes in gastric cancer tissue were significantly less than that of adjacent tissues.Patients with high CDK5 expression got higher immune gene ennchment scores(especially for the parainflammation and antigen presenting cell co-stimulation gene-sets).CDK5 expression is positively corof CDK5 and CD274 had a higher overall survival rate.IHC results showed that the number of CD8⁺T lymphocytes infiltrated in the CDK5 high expression group was significantly more than that m the CDK5 low expression group(P<0.01).Conclusion The expression of CDK5 in gastric cancer is related to the expression of CD274 and the infiltration of CD8⁺T lymphocytes,which may be involved in the immune escape of gastnc cancer.