一起家庭聚集性发热伴血小板减少综合征疫情的流行病学及病原学分析

目的 对一起家庭聚集性发热伴血小板减少综合征(SFTS)疫情的流行病学和病原学进行分析,为疾病防控及监测方案的制定提供参考.方法 对2例SFTS病例开展流行病学调查,对其居家周围的人群及宿主动物进行监测,通过酶联免疫吸附试验(ELISA)和实时荧光定量PCR方法对病例、动物血清及蜱进行血清学和病原学检测,对病例和蜱进行...

Epidemiological investigation and etiological analysis on a cluster of severe fever with thrombocytopenia syndrome in a family

  Objective   To analyze the epidemiological characteristics of a cluster of severe fever with thrombocytopenia syndrome (SFTS) in a family, and provide the basic support for the disease prevention, control and surveillance.   Methods   The epidemiological survey of 2 suspected fever cases were conducted, the healthy people and host animals in the nearby environment were monitored with serologic and etiologic methods. ELISA and real-time PCR were applied to detect the blood samples from the cases or animals and ticks samples. The nucleic acid positive samples were used for the sequencing of S, M and L genes of SFTS virus. The phylogenetic tree was constructed to analyze the association between the cases and the ticks   Results   Two cases were SFTS virus IgM and total antibody positive, and one case was positive for nucleic acid of SFTS virus. Three of 45 healthy human serum samples were positive for SFTS virus antibody. In the host animals, 6 dogs (including cases’ domesticated dog) and four sheep were total antibody positive, while four pigs were negative. In 10 tick samples, SFTS virus was detected in 1 tick sample from the domesticated dog of the cases. Sequencing analysis showed that the S, M and L gene nucleotide homology of the cases and the tick was 99.9%–100.0%.   Conclusion   The cluster of this SFTS in a family was highly associated with the domesticated dog and parasitic ticks on the dog.