宏基因组测序在腹泻暴发调查中的应用

目的 评价宏基因组测序技术在腹泻暴发中的潜在应用价值.方法 收集2017年报告的2例聚集性腹泻暴发事件的样品,针对样品进行多重荧光PCR检测;同时,应用宏基因组测序技术对样品进行测序,基于测序数据分析暴发事件的病原体.结果 案例1采用多重荧光PCR检测,仅检测到副溶血弧菌;而采用宏基因组测序技术除检测到副溶血弧菌外,还...

Application of metagenome sequencing in investigation of diarrhea outbreak

  Objective  To evaluate the potential application value of metagenome sequencing in the investigation of diarrhea outbreak.  Methods  The samples of two diarrhea outbreaks reported in 2017 were collected and detected by multiplex fluorescent PCR. Meanwhile, the isolates were sequenced by metagenome sequencing, and the potential pathogens of the outbreaks were analyzed based on the sequencing data.  Results  For outbreak 1, only Vibrio parahaemolyticus was detected by multiplex fluorescent PCR, while Salmonella with higher abundance was detected by metagenome sequencing in addition to V. parahaemolyticus. For outbreak 2, Shigella was detected by metagenome sequencing in samples with no pathogen detected by multiplex fluorescent PCR.  Conclusion  Metagenome sequencing needs no prediction of potential pathogens in samples and can avoid misdiagnosis, which is more conducive to the detection of mixed infection. Moreover, it has higher detection sensitivity compared with fluorescent PCR under the sufficient sequencing depth.