人巨细胞病毒感染对早孕绒毛细胞Cyclins表达的影响及其机制的初步探讨

目的:探讨人巨细胞病毒(human cytomegalovirus,HCMV)感染孕妇后致宫内感染的机制。方法:检测15例血清HCMV IgM(+)早孕妇女绒毛中HCMVmRNA的表达;检测HCMVmRNA表达阳性的绒毛原位杂交蜕膜中诱生型一氧化氮合酶(induced ni-tric oxide sythnase,iNOS)mRNA的表达,用免疫组化技术检测细胞周期蛋白(Cyclins)D、E、B、A蛋白的表达,用生化方法测定绒毛组织中的超氧化物歧化酶(superoxide dis-mutase,SOD)、丙二醛(malondialdehyde,MDA);以同期血清HCMV IgM(-)、绒毛HCMVmRNA(-)的15例孕妇绒毛为对照组。结果:(1)15例血清HCMV IgM(+)孕妇中5例绒毛组织HCMVmRNA阳性,宫内传播率为33.33%;(2)5例HCMV-mRNA阳性绒毛Cy-clin D、Cyclin E表达发生率均较对照组高(80%vs 7.1%;80%vs 15.5%);而两组均无Cyclin A及Cyclin B表达;(3)HCMV-mRNA阳性蜕膜中iNOS-mRNA呈强表达,其吸光度为0.5021±0.0189显著高于对照组的0.1860±0.0119,差异有统计学意义(P<0.05);(4)HCMV mRNA阳性绒毛中SOD、MDA均高于对照组(178.35±13.25 vs 102.27±10.12;22.18±5.02 vs 10.15±4.56),(P均<0.05)。结论:孕早期绒毛活动性HCMV感染可导致Cyclin异常表达,NO、SOD、MDA对Cyclin的异常表达起了重要的作用。

Study on cyclins expression in chorionic villi of HCMV infection during early pregnancy and the mechanism of intrauterine HCMV infection

Objective:To investigate mechanism of teratogenesis in pregnant women with infected by HCMV(human cytomegalovirus).Methods:HCMV-IgM in maternal sera was evaluated by ELISA and HCMV-mRNA in chorionic villi was determined by RT-PCR.For subjects with both HCMV-IgM(+) and HCMV-mRNA(+),iNOS-mRNA in their decidua was detected through hybridization in situ.Furthermore,cyclins(D,E,B,A) were tested by immohistochemical test and SOD and MDA were determined by biochemical assay in chorionic villi.Meanwhile,15 women were studied as control who had healthy pregnancy and HCMV-IgM(-) in sera,HCMV-mRNA(-) in chorionic villi.Results:(1)Fifteen pregnant women were HCMV-IgM(+) in serum of whom five also had result of HCMV-mRNA(+) in chorionic villi.The intrauterine transmission rate was 33.33%.(2) Five patients with HCMV-mRNA(+) in chorionic villi had more frequency of cyclin D and E than that of control group(80% vs 7.1%,80% vs 15.5%).However,cyclin A,cyclin B wasn't detected in both of them.(3) iNOS-mRNA had statistically higher level in decidual samples with HCMV-mRNA(+) than that of control group(0.5021±0.0189 vs 0.1860±0.0119,P<0.05).(4)Chorionic villi with HCMV-mRNA(+) showed elevated levels of SOD and MDA with statistical difference(P<0.05) in comparison of control group.Conclusion:Expressions of cyclins in chorionic villi are altered in active HCMV infection during early pregnancy in which elevated-level NO,SOD and MDA may play important roles.