乳腺癌患者血清血管内皮生长因子的表达及临床意义

目的 研究血管内皮生长因子在乳腺癌患者周围血血清中的表达与乳腺癌临床病理特征及乳腺癌转移复发之间的关系。方法 用ELISA酶联免疫吸附测定法定量检测31例乳腺癌术前血清以及其中12例乳腺癌术后随访时血清VEGF含量。结果 乳腺癌组血清VEGF含量与良性肿瘤对照组、正常对照组比较，差异均有显著性；乳腺癌术前血清VEGF含量与临床分期、有无腋窝淋巴结转移及远处转移有相关性；乳腺癌术前血清VEGF含量与乳腺癌肿瘤直径大小、4种乳腺癌肿瘤组织类型无相关性；乳腺癌患者术后随访时新出现转移者与无复发转移者的血清VEGF含量及两者术前血清VEGF含量差异均有统计学意义。结论 乳腺癌患者术前血清VEGF含量可作为鉴别诊断乳腺良恶性肿痛，预测乳腺癌分期、有无转移及术前化疗的独立指标之一；术后定期监测血清VEGF含量变化可用于判断乳腺癌复发及治疗效果。

Expression of vascular endothelial growth factor in blood serum of patients with human breast carcinoma and its clinical significance

Objective] To evaluate the expression of vascular endothelial growth factor (VEGF) in blood serum and its relation to metastasis and prognosis significance in breast cancer. Methods] The level of VEGF in blood serum was detected in 31 cases of breast carcinomas pre-operation and 12 cases of breast carcinomas after-operation by the method of enzyme-linked immunosorbent assay(ELISA). Results] The concentration of VEGF in blood serum difference among breast carcinoma, breast benign tumor and normal women was significant. The concentration of VEGF in blood serum of breast carcinoma was closely related to clinical stage, axillary lymph node metastasis, distant metastasis. There was no significant relationship between the concentration of VEGF in blood serum of breast carcinoma and histological type or tumor diameter. In follow-up study after operation, the concentration of VEGF in blood serum of breast carcinoma which had tumor metastasis and recurrence after operation was significantly higher than that of no tumor metastasis and recurrence. Before operation, the concentration of VEGF in blood serum of breast carcinoma was higher in the cases with tumor metastasis and recurrence after operation, than in those with no tumor metastasis and recurrence after operation. Conclusions] The VEGF levels in blood serum of breast carcinoma can be conducted as an assisted marker to differential diagnosis of breast tumor binding screenage results, it also can be conducted as an independent marker to predict the clinical TNM stage, tumor metastasis and necessary chemotherapy. It is useful for judgeing breast carcinoma recurrence and treament effects to monitor the VEGF level in blood serum of breast carcinoma after operation periodicly.