| 高纯度木瓜蛋白酶的分离纯化和性质研究 |
| |
| 引用本文： | 王丽彬,张弢,王旻.高纯度木瓜蛋白酶的分离纯化和性质研究[J].中国生化药物杂志,2006,27(3):159-162. |
| |
| 作者姓名： | 王丽彬 张弢 王旻 |
| |
| 作者单位： | 中国药科大学,生命科学与技术学院,江苏,南京,210009 |
| |
| 摘 要： | 目的建立木瓜蛋白酶的分离纯化工艺路径。方法采用初步采集处理,20%,40%硫酸铵分级沉淀、SP-sephadex C50柱色谱、羟基磷灰石柱色谱,从番木瓜乳汁中分离纯化木瓜蛋白酶。结果获得比活为1 184 U/mg的纯酶,活力回收率为55.79%,纯化的酶经SDS-PAGE呈单一条带,高效液相色谱呈单一条带,纯度达到99.31%。该酶耐高温,最适pH值为7.2,对酪蛋白的动力学常数(Km)为3.5×10-5mol/L。结论应用此方法成功纯化了高纯度的木瓜蛋白酶。
|
| 关 键 词： | 木瓜蛋白酶 木瓜 纯化 酶学性质 |
| 文章编号： | 1005-1678（2006）03-0159-04 |
| 收稿时间： | 2006-01-06 |
| 修稿时间： | 2006-02-22 |
Purification and characterization of high purity papain from Carica papaya |
| |
| WANG Li-bin,ZHANG Tao,WANG Min.Purification and characterization of high purity papain from Carica papaya[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(3):159-162. |
| |
| Authors: | WANG Li-bin ZHANG Tao WANG Min |
| |
| Abstract: | Purpose To construct a simple way for purifying papain from Carica papaya.MethodsUsing means of collection,20% and 40% saturated ammonium sulfate fractionation,SP-sephadex C 50 column chromatography and hydroxyapatite chromatography to purify papain from Carica papaya.ResultsA 55.79% yield of enzyme with a specific activity of 1 184 U/mg was obtained.The purified enzyme shows a single band on SDS-PAGE and a purity of 99.31% on HPLC.The enzyme is able to bear high temperature,and its optimum pH is 7.2,the K_m with casein is 3.5×10~(-5) mol/L.ConclusionPapain was successfully purificated by a simple method. |
| |
| Keywords: | papain Carica papaya purification enzyme characterization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
